Islet Metabolism Core

目的：DERC胰岛代谢中心帮助目前正在研究或计划研究胰岛细胞生物学、移植和代谢分析的研究人员。 组成部分： 1.胰岛纯化。用于胰岛移植、基因组研究和细胞生物学研究的人类和小鼠胰岛的处理和制备。 2.胰岛功能分析。对人和小鼠胰岛的胰岛素产生和其他功能方面进行真实的实时生物和生化分析，以表征不同条件下的胰岛活性，并评估用于移植研究的胰岛制备质量。 3.小鼠代谢分析。分析完整小鼠的代谢状态，包括测量脂肪含量、监测响应于外源性葡萄糖或胰岛素刺激的葡萄糖水平以及分析氧消耗和/或活动。 对DERC社区的好处： 这些高度专业化的能力是几乎所有单个实验室所无法企及的。胰岛代谢核心整合了这些独特的功能，并使这些功能能够应用于DERC研究人员的研究。标准化的方案确保了在不同日期进行的分析的重现性。标准化还使各个实验室能够比较结果。 技术开发： 有一个持续的承诺，继续在胰岛分离和分析先前的改进。所有准备者的胰岛功能标准的更广泛的检查将与核心范围的数据库相结合，以帮助研究者进行交叉研究推断。完整小鼠代谢分析的显著改善预计将代表下一个DERC周期的另一项重大努力。

Purpose: The DERC Islet Metabolism Core assists investigators who currently study or have plans to study pancreatic islet cell biology, transplantation and metabolic analysis. Components: 1. Islet Purification. Processing and preparation of human and mouse islets for use in islet transplantation, genomic studies, and cell biology studies. 2. Islet Functional Analysis. Real time biological and biochemical analysis of insulin production and other functional aspects of human and mouse islets to characterize islet activity under different conditions and to assess islet preparation quality for transplantation studies. 3. Mouse Metabolic Analysis. Analysis of the metabolic status of an intact mouse including measurements of fat content, the monitoring of glucose levels in response to exogenous glucose or insulin stimulation and the analysis of oxygen consumption and/or activity. Benefits to DERC Community: These highly specialized capabilities are beyond the reach of almost ail individual laboratories. The Islet Metabolism Core consolidates these unique capabilities and enables the application of these capabilities to research studies by DERC investigators. Standardized protocols ensure reproducibility in analyses conducted on different days. Standardization also enables individual laboratories to compare outcomes. Technology Development: There is an ongoing commitment to continue the prior improvements in islet isolation and analysis. A broader work-up of islet function standard for all preps will be combined with a Core-wide database to assist investigators in making cross-study inferences. Significant improvements in the metabolic analysis of intact mice are expected to represent another major effort in the next DERC cycle.