LuxS-Mediated Quorum Sensing in Streptococcus mutans

LuxS 介导的变形链球菌群体感应

基本信息

  • 批准号:
    6760192
  • 负责人:
  • 金额:
    $ 7.27万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2003
  • 资助国家:
    美国
  • 起止时间:
    2003-06-15 至 2006-05-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Streptococcus mutans is recognized as the principal etiological agent of dental caries, the most prevalent infectious disease of humans. The ability to metabolize carbohydrates and generate acids, to survive acidic pH and other adverse conditions, and to adhere to and form tenacious biofilms on the tooth surface are believed to be critically associated with the cariogenicity of this human pathogen. Known for its high degree of acid tolerance (aciduricity) and its high capacity to produce acid (acidogenicity), S. mutans lives primarily on the tooth surface at high cell-density in a high diversity ecosystem better known as dental plaque, the structure and composition of which is known to be largely influenced by such factors as the source and availability of nutrients, the pH in the oral cavity and by the ability of the biofilm organisms to adapt to the fluctuations in environmental conditions. Quorum sensing is a cell density--dependent regulatory mechanism that is known to be involved in regulation of a variety of physiologic processes and virulence in both Gram (+) and Gram (-) bacteria. We have recently generated evidence that the S. mutans possesses a gene encoding a functional homologue of the new family of autoinducer synthases (LuxS) that are responsible for production of autoinducers of the quorum sensing system 2, AI-2. This study is designed to yield novel information concerning LuxS-mediated quorum sensing and virulence regulation in S. mutans, which will contribute to our understanding of the pathogenesis of this microorganism and the ecology of the oral flora. The Specific Aims of this proposed study are: 1) to investigate the role of luxS in acid tolerance by S. mutans. By using functional assays, reporter gene fusions, Northern hybridization, and proteomics, we will investigate acid tolerance and its regulation by luxS, and identify novel factors (proteins) that are involved in luxS-regulated acid tolerance responses. 2). To use confocal laser scanning microscopy (CLSM) and mixed, known-species consortia to determine the impact of luxS of S. mutans on bacterial adherence by S. mutans and the inter- and intra-generic interactions between S. mutans and other oral bacteria in terms of biofilm initiation development and structure.
描述(申请人提供):变形链球菌被认为是龋齿的主要病原体,龋齿是人类最常见的传染病。代谢碳水化合物和产生酸的能力,在酸性pH值和其他不利条件下生存的能力,以及在牙齿表面粘附并形成坚韧的生物膜的能力,被认为与这种人类病原体的蛀牙性密切相关。变形链球菌以其高度的耐酸性(酸性)和高产酸能力(致酸性)而闻名,主要生活在牙齿表面,以高细胞密度生活在一个高度多样化的生态系统中,即牙菌斑,其结构和组成在很大程度上受营养来源和可用性等因素的影响。口腔内的pH值以及生物膜生物适应环境条件波动的能力。群体感应是一种依赖于细胞密度的调节机制,已知参与了革兰氏(+)和革兰氏(-)细菌的多种生理过程和毒力的调节。我们最近有证据表明,变形链球菌拥有一个基因,编码新的自诱导合成酶家族(LuxS)的功能同源物,该家族负责产生群体感应系统2 (AI-2)的自诱导物。本研究旨在获得关于突变链球菌中luxs介导的群体感应和毒力调节的新信息,这将有助于我们了解这种微生物的发病机制和口腔菌群的生态学。本研究的具体目的是:1)研究luxS在变形链球菌耐酸中的作用。通过功能分析、报告基因融合、Northern杂交和蛋白质组学,我们将研究luxS的耐酸性及其调控,并确定参与luxS调控的耐酸反应的新因子(蛋白质)。2). 利用共聚焦激光扫描显微镜(CLSM)和混合已知菌群研究变形链球菌luxS对细菌粘附的影响,以及变形链球菌与其他口腔细菌在生物膜起始、发育和结构方面的属间和属内相互作用。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

ZEZHANG TOM WEN其他文献

ZEZHANG TOM WEN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('ZEZHANG TOM WEN', 18)}}的其他基金

More Than Mechanical Retention: Characterization of Lactobacillus Clinical Strains Using In Vitro Models
不仅仅是机械保留:使用体外模型表征乳酸菌临床菌株
  • 批准号:
    10593599
  • 财政年份:
    2023
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    9385105
  • 财政年份:
    2016
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    8282949
  • 财政年份:
    2009
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    7741391
  • 财政年份:
    2009
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    9314529
  • 财政年份:
    2009
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    10539743
  • 财政年份:
    2009
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    10661800
  • 财政年份:
    2009
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    8089432
  • 财政年份:
    2009
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    7821444
  • 财政年份:
    2009
  • 资助金额:
    $ 7.27万
  • 项目类别:
BrpA in Virulence Modulation of Streptococcus mutans
BrpA 在变形链球菌毒力调节中的作用
  • 批准号:
    9118974
  • 财政年份:
    2009
  • 资助金额:
    $ 7.27万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了