Microbial sunscreens for better life

微生物防晒霜让生活更美好

• 批准号: 2433040
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2433040
• 关键词: Microbial; sunscreens; better; life

Cases of cosmeceuticals interfering with marine life are now widespread. This is largely due to increasing societal trends towards exposure to ultraviolet radiation (UVR) from sunlight. Sunscreens provide UVR-protection; however, this often comes at a cost as the desired sun protection factor (SPF) is achieved with high concentrations of UVR filters within a blend. This can lead to serious environmental issues. For example, some sunscreens are known to be damaging to marine life [1,2]; indeed Hawaii, will ban the use of certain UVR filters (e.g. oxybenzone) in sunscreen blends due to coral bleaching [3]. It is therefore key to develop next generation, safer sunscreens which preserve the quality of aquatic life.The proposed CENTA2 project seeks to answer the overarching question: Can microorganism (e.g. algae, cyanobacteria etc.)-based UVR filters be chemically modified for human use, that combine the resilience to UVR exposure and, importantly to the ethos of NERC, are much safer to the environment? Three researchers within the University of Warwick (UoW) spanning Chemistry, Life Sciences and Centre for Scientific Computing, along with Lubrizol, a leading skin-care industry, propose to apply their complementary skills in experiment and theory to train a highly talented early career researcher to confront the multi-facetted challenges imposed by this question.The proposed programme offers a potentially transformative contribution to the photophysically fascinating, and marine (and health)-care vital, field of sunscreen science. The present CENTA2 is an ideal platform to support this research, given the closely aligned links between NERC Research Areas including: (1) Pollution, Waste and Resources (closely aligned); (2) Marine Environments; and (3) Environmental Microbiology, as well as being intimately aligned with one of the Sciences Themes of CENTA2, that of Climate and Environmental Sustainability. Lastly, the present project provides an ideal opportunity to link with Lubrizol's division of Skin Care, led by Professor Laurent Blasco, Honorary Professor at the University of Warwick and Lubrizol's global skin care manager.Methodology:Theory and computation: steady-state calculations will employ TD-DFT [4,5]/CASPT2 [6,7] to yield details of molecular structure of UVR filters, while dynamics simulations will use trajectory surface hopping methods and solvation models to compute photorelaxation dynamics in solution/condensed- phases.Biosynthesis: The UVR filters will involve bacterial expression, purification and characterisation. Bacterial production of these nature-derived systems will be based on well established procedures as discussed in REFS [8] and [9].Spectroscopy: Transient absorption spectroscopy [10] will be used to track energy flow in these molecules following absorption of UVR. These techniques are crucial in enabling us to build molecular- movies of energy flow over the time window of 10-15-10-3 s.Analytical chemistry: The most promising UVR filters will be converted to a sunscreen blend (UVR-filter/moisturizer) and deposited on skin model VITRO-SKIN [11]. Following UVR exposure, the blend will be washed off in water and contents analysed for potential photogenerated/phototoxic products

药妆品干扰海洋生物的案例现在很普遍。这主要是由于社会越来越倾向于暴露于阳光中的紫外线辐射 (UVR)。防晒霜提供紫外线防护；然而，这通常是有代价的，因为所需的防晒系数 (SPF) 是通过混合物中高浓度的 UVR 过滤剂来实现的。这可能导致严重的环境问题。例如，已知某些防晒霜会对海洋生物造成损害[1,2]；事实上，由于珊瑚白化，夏威夷将禁止在防晒霜混合物中使用某些 UVR 过滤剂（例如氧苯酮）[3]。因此，开发下一代更安全的防晒霜来保护水生生物的质量是关键。拟议的 CENTA2 项目旨在回答一个首要问题：基于微生物（例如藻类、蓝细菌等）的 UVR 过滤剂是否可以经过化学修饰以供人类使用，既具有对 UVR 暴露的抵抗力，又重要的是符合 NERC 的精神，对环境更加安全？华威大学 (UoW) 化学、生命科学和科学计算中心的三名研究人员与领先的皮肤护理行业路博润一起，提议运用他们在实验和理论方面的互补技能来培养一名才华横溢的早期职业研究人员，以应对这个问题带来的多方面挑战。拟议的项目为光物理和海洋（和海洋）领域提供了潜在的变革性贡献。 健康）-护理至关重要，防晒科学领域。鉴于 NERC 研究领域之间的紧密联系，目前的 CENTA2 是支持这项研究的理想平台，包括： (1) 污染、废物和资源（紧密结合）； (2) 海洋环境； (3) 环境微生物学，并与 CENTA2 的科学主题之一即气候和环境可持续性密切相关。最后，本项目提供了与路博润皮肤护理部门联系的理想机会，该部门由华威大学名誉教授兼路博润全球皮肤护理经理 Laurent Blasco 教授领导。方法论：理论和计算：稳态计算将采用 TD-DFT [4,5]/CASPT2 [6,7] 来生成 UVR 过滤器分子结构的详细信息，而动态模拟将使用轨迹 表面跳跃方法和溶剂化模型来计算溶液/凝聚相中的光弛豫动力学。生物合成：UVR过滤器将涉及细菌表达、纯化和表征。这些源自自然的系统的细菌生产将基于 REFS [8] 和 [9] 中讨论的完善程序。光谱：瞬态吸收光谱 [10] 将用于跟踪吸收 UVR 后这些分子中的能量流。这些技术对于我们能够在 10-15-10-3 秒的时间窗口内构建能量流的分子电影至关重要。分析化学：最有前途的 UVR 过滤器将被转换为防晒混合物（UVR 过滤器/保湿剂）并沉积在皮肤模型 VITRO-SKIN 上[11]。紫外线照射后，混合物将在水中被冲洗掉，并分析潜在的光生/光毒性产物的含量