Fastigial nucleus role in long-term saccade adaptation

描述（由申请人提供）：拟议的研究将表征来自尾顶核（CFN）的扫视相关小脑输出在长期扫视适应中的作用。小脑与短期扫视适应密切相关，但其在长期扫视适应中的作用尚不清楚。长期适应尚未得到广泛研究，但对于在生长、衰老过程中保持运动准确性以及受损运动的永久康复至关重要。长期的适应性眼跳将产生的适应性刺激，以一只猴子许多天。在第一个实验中，行为测试和暂时失活的CFN将被用来测试我们的假设，小脑的贡献，以适应扫视后迅速下降的初步适应。在第二个实验中，永久性双侧CFN病变将被用来测试我们的假设，CFN输出驱动长期适应。在第三个实验中，在CFN中注射顺行示踪剂，在额眼区（FEF）中注射逆行示踪剂将检验我们的假设，即CFN输出通过丘脑中的中继到达FEF。该通路可能通过修改从FEF下降的扫视命令来诱导长期适应。本研究有三个具体的目的：（1）测量CFN在长期适应过程中不同时间对适应性眼跳的贡献。(2)测量双侧CFN永久性毁损后的长期适应性。（3）确定长期适应性的解剖学途径。

DESCRIPTION (provided by applicant): The proposed research will characterize the role of saccade-related cerebellar output from the caudal fastigial nucleus (CFN), in long-term saccade adaptation. Cerebellum is strongly implicated in short-term saccade adaptation, but its role in long-term saccade adaptation is unknown. Long-term adaptation has not been extensively studied, but is essential for maintaining movement accuracy during growth, aging, and for permanent rehabilitation of impaired movements. Long-term adaptation of saccades will be produced by presenting an adapting stimulus to a monkey for many days. In the first experiment, behavioral tests and temporary inactivation of the CFN will be used to test our hypothesis that cerebellar contribution to adapted saccades decreases rapidly after the initial adaptation. In a second experiment, permanent bilateral CFN lesions will be used to test our hypothesis that CFN output drives long-term adaptation. In third experiment, injections of anterograde tracer in the CFN, and retrograde tracer in the frontal eye field (FEF) will test our hypothesis that CFN output reaches the FEF via a relay in the thalamus. This pathway may induce long-term adaptation by modifying saccade commands descending from the FEF. The proposed research has three specific aims: (1) To measure the contribution of CFN to adapted saccades at different times during long-term adaptation. (2) To measure the long-term adaptation after permanently lesioning the CFN bilaterally (3) To determine the anatomical pathway of Iong-term adaptation.