Fastigial nucleus role in long-term saccade adaptation

描述（由申请人提供）：拟议的研究将描述尾顶核（CFN）与眼跳相关的小脑输出在长期眼跳适应中的作用。小脑与短期眼跳适应密切相关，但其在长期眼跳适应中的作用尚不清楚。长期适应尚未得到广泛研究，但对于在生长、衰老过程中保持运动准确性以及运动受损的永久康复至关重要。通过向猴子提供多天的适应刺激，可以产生眼跳的长期适应。在第一个实验中，行为测试和 CFN 的暂时失活将用于检验我们的假设，即小脑对适应眼跳的贡献在初始适应后迅速下降。在第二个实验中，永久性双侧 CFN 损伤将用于检验我们的假设，即 CFN 输出驱动长期适应。在第三个实验中，在 CFN 中注射顺行示踪剂，在额眼场 (FEF) 中注射逆行示踪剂将检验我们的假设，即 CFN 输出通过丘脑中的继电器到达 FEF。该途径可以通过修改来自 FEF 的扫视命令来诱导长期适应。拟议的研究有三个具体目标：（1）测量CFN在长期适应过程中不同时间对适应眼跳的贡献。 (2) 测量双侧 CFN 永久损伤后的长期适应 (3) 确定长期适应的解剖途径。

DESCRIPTION (provided by applicant): The proposed research will characterize the role of saccade-related cerebellar output from the caudal fastigial nucleus (CFN), in long-term saccade adaptation. Cerebellum is strongly implicated in short-term saccade adaptation, but its role in long-term saccade adaptation is unknown. Long-term adaptation has not been extensively studied, but is essential for maintaining movement accuracy during growth, aging, and for permanent rehabilitation of impaired movements. Long-term adaptation of saccades will be produced by presenting an adapting stimulus to a monkey for many days. In the first experiment, behavioral tests and temporary inactivation of the CFN will be used to test our hypothesis that cerebellar contribution to adapted saccades decreases rapidly after the initial adaptation. In a second experiment, permanent bilateral CFN lesions will be used to test our hypothesis that CFN output drives long-term adaptation. In third experiment, injections of anterograde tracer in the CFN, and retrograde tracer in the frontal eye field (FEF) will test our hypothesis that CFN output reaches the FEF via a relay in the thalamus. This pathway may induce long-term adaptation by modifying saccade commands descending from the FEF. The proposed research has three specific aims: (1) To measure the contribution of CFN to adapted saccades at different times during long-term adaptation. (2) To measure the long-term adaptation after permanently lesioning the CFN bilaterally (3) To determine the anatomical pathway of Iong-term adaptation.