Fastigial nucleus role in long-term saccade adaptation

描述(由申请人提供)：拟议的研究将表征与眼跳相关的小脑输出从尾侧顶核(CFN)在长期眼跳适应中的作用。小脑与短期眼跳适应密切相关，但其在长期眼跳适应中的作用尚不清楚。长期适应还没有得到广泛的研究，但对于在生长、衰老过程中保持运动的准确性以及对受损运动的永久康复来说是必不可少的。通过向猴子提供多天的适应刺激，将产生长期的眼跳适应。在第一个实验中，行为测试和CFN的暂时失活将被用来检验我们的假设，即小脑对适应眼跳的贡献在初始适应后迅速下降。在第二个实验中，永久性的双侧CFN损伤将被用来检验我们的假设，即CFN输出驱动长期适应。在第三个实验中，在CFN注射顺行示踪剂，在额叶眼野(FEF)注射逆行示踪剂，将检验我们的假设，即CFN的输出通过丘脑的一个中继站到达FEF。这一途径可能通过改变从FEF下传的眼跳指令来诱导长期适应。本研究有三个具体目的：(1)在长期适应的不同时间测量CFN对适应眼跳的贡献。(2)测量双侧CFN永久性损伤后的长期适应；(3)确定长期适应的解剖学途径。

DESCRIPTION (provided by applicant): The proposed research will characterize the role of saccade-related cerebellar output from the caudal fastigial nucleus (CFN), in long-term saccade adaptation. Cerebellum is strongly implicated in short-term saccade adaptation, but its role in long-term saccade adaptation is unknown. Long-term adaptation has not been extensively studied, but is essential for maintaining movement accuracy during growth, aging, and for permanent rehabilitation of impaired movements. Long-term adaptation of saccades will be produced by presenting an adapting stimulus to a monkey for many days. In the first experiment, behavioral tests and temporary inactivation of the CFN will be used to test our hypothesis that cerebellar contribution to adapted saccades decreases rapidly after the initial adaptation. In a second experiment, permanent bilateral CFN lesions will be used to test our hypothesis that CFN output drives long-term adaptation. In third experiment, injections of anterograde tracer in the CFN, and retrograde tracer in the frontal eye field (FEF) will test our hypothesis that CFN output reaches the FEF via a relay in the thalamus. This pathway may induce long-term adaptation by modifying saccade commands descending from the FEF. The proposed research has three specific aims: (1) To measure the contribution of CFN to adapted saccades at different times during long-term adaptation. (2) To measure the long-term adaptation after permanently lesioning the CFN bilaterally (3) To determine the anatomical pathway of Iong-term adaptation.