Fastigial nucleus role in long-term saccade adaptation

描述（由申请人提供）：拟议的研究将描述与眼跳相关的小脑输出来自尾侧纹状核（CFN）在长期眼跳适应中的作用。小脑与短期扫视适应密切相关，但其在长期扫视适应中的作用尚不清楚。长期适应尚未得到广泛的研究，但对于在生长、衰老过程中保持运动准确性以及运动受损的永久康复至关重要。长期的眼跳适应将通过向猴子提供适应刺激而产生。在第一个实验中，将使用行为测试和暂时失活CFN来验证我们的假设，即小脑对适应性扫视的贡献在初始适应后迅速下降。在第二个实验中，永久性双侧CFN病变将用于验证我们的假设，即CFN输出驱动长期适应。在第三个实验中，我们在前眼区注射顺行示踪剂，在额眼区（FEF）注射逆行示踪剂，以验证我们的假设，即CFN输出通过丘脑中的中继到达前眼区。这一途径可能通过修改从FEF下行的扫视指令来诱导长期适应。本研究有三个具体目标：(1)衡量长期适应过程中不同时期CFN对适应跃迁的贡献。(2)测量CFN双侧永久性损伤后的长期适应(3)确定长期适应的解剖学途径。

DESCRIPTION (provided by applicant): The proposed research will characterize the role of saccade-related cerebellar output from the caudal fastigial nucleus (CFN), in long-term saccade adaptation. Cerebellum is strongly implicated in short-term saccade adaptation, but its role in long-term saccade adaptation is unknown. Long-term adaptation has not been extensively studied, but is essential for maintaining movement accuracy during growth, aging, and for permanent rehabilitation of impaired movements. Long-term adaptation of saccades will be produced by presenting an adapting stimulus to a monkey for many days. In the first experiment, behavioral tests and temporary inactivation of the CFN will be used to test our hypothesis that cerebellar contribution to adapted saccades decreases rapidly after the initial adaptation. In a second experiment, permanent bilateral CFN lesions will be used to test our hypothesis that CFN output drives long-term adaptation. In third experiment, injections of anterograde tracer in the CFN, and retrograde tracer in the frontal eye field (FEF) will test our hypothesis that CFN output reaches the FEF via a relay in the thalamus. This pathway may induce long-term adaptation by modifying saccade commands descending from the FEF. The proposed research has three specific aims: (1) To measure the contribution of CFN to adapted saccades at different times during long-term adaptation. (2) To measure the long-term adaptation after permanently lesioning the CFN bilaterally (3) To determine the anatomical pathway of Iong-term adaptation.