Marine and Freshwater Biomedical Sciences Center

描述（由申请人提供） 该提案旨在为NIEHS海军陆战队提供持续资金， 迈阿密大学淡水生物医学科学中心。地基于 海洋与大气科学学院Rosenstiel School of Marine and Atmospheric Sciences Campus 来自迈阿密三所大学的19名调查人员共同努力， 校园和6个外部组织。两个主要的研究主题构成了 跨学科研究核心的基础：“海洋和淡水毒素， 海洋和淡水动物模型毒素和人类 健康“。这两个研究主题都在NIEHS赞助的范围内。 研究和促进迈阿密大学的整体使命 海洋和淡水生物医学科学中心：评估 海洋和淡水水体对人类健康的影响， 了解风险，并寻求补救措施。第一个研究核心 包括：毒素生物合成和探针开发，代谢，分子 药理学，分子建模，电生理学受体结合， 分离技术和分析方法，流行病学和公共卫生。 第二个研究核心包括几个海洋和淡水（和人类） 不同发育阶段的模型：雀鲷神经纤维瘤病， 培养的人雪旺氏细胞，失智症神经生理学，蟾蜍 高氨血症，转基因鱼，松鼠鱼锌代谢/转运， 鱼类免疫学和哨兵物种。一个充满活力的试点项目计划是 代表四个最近选定的应用程序：“功能分析的 转基因斑马鱼中的锌调控基因”;“赤潮毒素对斑马鱼的影响” 听觉：脊椎动物模型”;“致癌物的分子传感器 污染物”和“亚热带微生物再生水指标” 海洋环境”。支持现有的个人和合作 方案，4个设施和服务核心提出了基于 调查员？项目利用率和整体价值评价 过去五年：毒素探针，供应短毒素，石房蛤毒素，冈田酸， 软骨藻酸、雪卡毒素和应用相关毒素衍生物，以及 毒素生物体的培养物，以及来自这些生物体的基于DNA的材料; 提供体外和体内鱼类养殖设施和专业知识， 鱼类和无脊椎动物细胞培养的维持和试验 分析化学和电子显微镜， 提供NMR、MS和分析分离技术协助， 几种EM方法;神经生理学，它提供了两个独立的 设备齐全的电生理设备以及额外的组织培养 支持能力。一个完善的社区外展和教育方案 将继续，包括中毒控制热线海鲜中毒， NIEHS赞助的K-12教育计划，以及NIEHS赞助的博士后 培训计划。迈阿密大学将继续致力于 中心以费用分摊的形式进行薪资支持、仪器配套 资金和教师启动包。通过合并拟议的NIEHS 支持和UM成本分担，调查人员将继续执行基本的 研究毒素和动物模型，并传达NIEHS信息， 科学界和非科学界。

DESCRIPTION (provided by applicant) This proposal is to provide continued funding of an NIEHS Marine and Freshwater Biomedical Sciences Center at the University of Miami. Based at the Rosenstiel School of Marine and Atmospheric Sciences campus, the Center is it collaborative effort of 19 investigators from three University of Miami campuses and 6 external organizations. Two principal research themes form the basis for interdisciplinary Research Cores: "Marine and Freshwater Toxins and Human Health " and "Marine and Freshwater Animal Models Toxins and Human Health ". Both research themes are within the scope of NIEHS-sponsored research and contribute to the overall mission of the University of Miami Marine and Freshwater Biomedical Sciences Center: to evaluate the impact of the oceans and freshwater bodies on human health, by assessing and understanding risks, and by seeking remedies. The first research core includes: Toxin Biosynthesis and Probe Development, Metabolism, Molecular Pharmacology, Molecular Modeling, Electrophysiology Receptor Binding, Separation Techniques and Assay Methods, and Epidemiology and Public Health. The second research core includes several marine and freshwater (and human) models in various stages of development: Damselfish Neurofibromatosis, Cultured Human Schwann Cells, Aplysia Neurophysiology, Toadfish Hyperammonemia, Transgenic Fishes, Squirrelfish Zinc Metabolisin/Transport, Fish Immunology arid Sentinel Species. A vigorous Pilot Project Program is represented by four recently selected applications on: "Functional Analysis of Zinc Regulatory Genes in Transgenic Zebrafish"; "Red Tide Toxin Effects on Hearing: a Vertebrate Model"; "Molecule-Based Sensors for Carcinogenic Pollutants"; and "Microbial Recreational Water Indicators in the Subtropical Marine Environment". In support of existing individual and collaborative programs, 4 Facilities and Service Cores are proposed based on the investigators? evaluation of utilization and overall value to programs in the past five years: Toxin Probes, supplying brevetoxins, saxitoxin, okadaic acid, domoic acid, ciguatoxins and application-related toxin derivatives, as well as cultures of toxin organisms, and DNA-based materials from these organisms; In vitro and In vivo Fish Culture supplying facilities and expertise or the maintenance of, and experimentation with, fish and invertebrate cell cultures and live organisms; Analytical Chemistry and Electron Microscopy, which provides NMR, MS and analytical separation techniques assistance, and access to several EM methodologies; Neurophysiology, which provides two separate fully-equipped electrophysiology rigs, as well as additional tissue culture support capability. A well developed Community Outreach and Education Program will continue, including a poison-control hotline for seafood intoxication, an NIEHS-sponsored K-12 education program, and an NIEHS-sponsored postdoctoral training program. University of Miami will continue its commitment to the Center in the form of cost-share for salary support, instrumentation matching funds, and a faculty start-up package. Through the combined proposed NIEHS support and UM cost share, the investigators will continue to perform basic research on toxins and animal models, and to communicate the NIEHS message to the scientific and lay communities.