Metal Ion Probes Membrane Protein Structure And Function

描述：(申请人提供)本提案的重点是 了解中特定结构/功能关系的基础 复杂的蛋白质反应--通过考察 来自不同生物体的“相同”蛋白质。这种方法将有助于理解 蛋白质结构、功能和其他性质的自然变异 在不同的亚型或不同的物种之间，是一种必要的补充 对单一蛋白质的定点突变的研究。这一努力是 对使用EPR作为结构性调查具有长期兴趣，并利用 开发和应用创新的EPR答疑方法的经验 具体的生物学和生理问题。 这项工作将提供对生物能量上重要的细胞色素的深入了解 BC型复合体与代谢重要的真核细胞色素P450 酶可能导致新的、高选择性的药物和抗生素 利用这些蛋白质在不同生物体中或不同生物之间的差异 在人类中有不同的异构体。开展这项工作所采用的方法 将广泛适用于其他膜蛋白和其他蛋白 复合体。

DESCRIPTION: (provided by applicant) The focus of this proposal is to understand the basis for the specific structure/function relationship in complex protein reactions by examining differences and similarities between the 'same' protein from different organisms. This approach will help to understand the natural variation in protein structure, function and other properties between different isoforms or different species and is a necessary complement to the study of site-directed mutants of a single protein. This effort is part of a long-term interest in the use of EPR as a structural probe and draws on experience in developing and applying innovative EPR methods for answering specific biological and physical questions. This work will provide insights into the bioenergetically important cytochrome bc-type complex and the metabolically important eukaryotic cytochrome P450 enzymes that could lead to new, highly-selective drugs and antibiotics exploiting differences between these proteins in different organisms or between different isoforms in humans. The methods developed in carrying out this work will be broadly applicable to other membrane proteins and other protein complexes.