Metal Ion Probes Membrane Protein Structure And Function

描述：（由申请人提供）本提案的重点是 理解特定结构/功能关系的基础， 复杂的蛋白质反应，通过检查之间的差异和相似之处， 不同生物体的“相同”蛋白质。这种方法将有助于理解 蛋白质结构、功能和其他性质的自然变异 在不同亚型或不同物种之间， 研究单一蛋白质的定点突变体。这一努力是 的长期利益，在使用EPR作为结构探针，并借鉴 开发和应用创新的EPR方法， 具体的生物和物理问题。 这项工作将提供深入了解生物能量重要的细胞色素 bc-型复合物和代谢重要的真核细胞色素P450 这些酶可能会导致新的，高选择性的药物和抗生素 利用不同生物体中这些蛋白质之间的差异， 不同的亚型。在开展这项工作时所采用的方法 将广泛适用于其它膜蛋白和其它蛋白质 配合物

DESCRIPTION: (provided by applicant) The focus of this proposal is to understand the basis for the specific structure/function relationship in complex protein reactions by examining differences and similarities between the 'same' protein from different organisms. This approach will help to understand the natural variation in protein structure, function and other properties between different isoforms or different species and is a necessary complement to the study of site-directed mutants of a single protein. This effort is part of a long-term interest in the use of EPR as a structural probe and draws on experience in developing and applying innovative EPR methods for answering specific biological and physical questions. This work will provide insights into the bioenergetically important cytochrome bc-type complex and the metabolically important eukaryotic cytochrome P450 enzymes that could lead to new, highly-selective drugs and antibiotics exploiting differences between these proteins in different organisms or between different isoforms in humans. The methods developed in carrying out this work will be broadly applicable to other membrane proteins and other protein complexes.