MAGNETIC RESONANCE UPDATE: PULSED EPR, ENDOR, ELDOR

说明(申请人提供)：本申请申请资金用于升级脉冲电子顺磁共振(EPR)光谱仪，该光谱仪是1993年购买的Bruker ESP 380E，以便在NIH的支持下继续支持七个研究项目，涉及六个不同机构的研究人员。我们试图通过获得一个现代化的控制台和一个带有Endor和Eldor附件的微波桥，将该仪器升级到新的最先进的ElexSys 580的水平。对于每个用户组，EPR测量要么是他们NIH授权的特定目标，要么是EPR测量将解决研究中关键路径上的问题。所要求的更新将对所有主要用户产生明显影响，详见个别用户说明。好处包括：在重复频率受到当前光谱仪硬件限制的系统中提高了灵敏度；消除了相干噪声和‘TX’噪声提高了灵敏度和分辨率；消除了在重要数据区域中间产生空白“窗口”的当前硬件限制；Endor、Triple和ENQOR谱的分辨率提高，其中分辨率目前受到脉冲宽度硬件限制的限制；由于计算机控制许多现在需要手动调整的光谱仪控制，更好地利用了夜间和周末运行；它将使使用脉冲序列和进行目前因光谱仪硬件和软件中的任意限制而无法进行的测量成为可能。

DESCRIPTION (provided by applicant): This application requests funds for the upgrade of a pulsed electron paramagnetic resonance (EPR) spectrometer, a Bruker ESP 380E purchased in 1993, in order to provide continued support for seven research projects with NIH support involving researchers at six different institutions. We seek to update this instrument to the level of a new state-of-the-art ElexSys 580 by obtaining a modern console and a microwave bridge with ENDOR and ELDOR accessories.For each user group, the EPR measurements are either a specific aim of their NIH grants or the EPR measurements will resolve a problem on the critical path in the research. The requested update will have clear impact on all of the major users as detailed in the individual user descriptions. The benefits include: Increased sensitivity in systems where the repetition rate is limited by the current spectrometer hardware; Increased sensitivity and resolution from the elimination of coherent noise and 'Tx' noise; Elimination of current hardware limitations that produce blank 'windows' in middle of important regions of data; Increased resolution in ENDOR, TRIPLE and ENQOR spectra where resolution is currently limited by hardware limitations on pulse width; Better utilization of overnight and weekend runs resulting from computer control of many spectrometer controls that now require manual adjustment; It will make it possible to employ pulse sequences and make measurements that are currently prevented by arbitrary limitations in the hardware and software of the spectrometer.