Monolayer Protected Nanoparticles Flavoenzyme Models

描述：(由申请人提供)场地隔离和提交 功能性是酶催化的两个关键要求。研究人员 已经开发出合成系统，有效地模仿 酶活性部位的功能或分离；构建物的创建 提供这两个属性的人仍然是一个巨大的挑战。我们会 在单分子膜保护的金簇上使用自组装单分子膜(SAM) (MMPC)以提供所需的隔离和预组织 有效复制黄素酶系统的行为。有三个关键 对我们提议的研究提出了以下几点： 1)酶-辅因子相互作用的有效复制 由SAM提供的预组织，加上由 MMPC侧链的径向性质。 2)制造提供有效分离辅因子的MMPC结构 埋藏在SAM中的溶剂和其他干扰物质。 3)我们将结合从上述计划中获得的专业知识来设计和 构建电子转移酶家族的全功能模拟物 黄素酶。这些调查将解决承认的关键问题 水和酶的控制模拟氧化还原电位，并将最终以 创造了一种功能强大的电子航天飞机，旨在完全复制 电子转移酶的功能。

DESCRIPTION: (provided by applicant) Site isolation and presentation of functionality are two key requirements for enzymatic catalysis. Researchers have developed synthetic systems that effectively mimic either the functionality or the isolation of enzyme active sites; creation of constructs that provide both of these attributes remains a significant challenge. We will use self-assembled monolayers (SAMs) on monolayer-protected gold clusters (MMPCs) to provide both the isolation and preorganization required to effectively replicate the behavior of flavoenzyme systems. There are three key thrusts to our proposed research: 1) The effective duplication of enzyme-cofactor interactions using the preorganization provided by the SAM, coupled with the control provided by the radial nature of the MMPC sidechains. 2) Fabrication of MMPC structures that provide effective isolation of cofactors buried within the SAM from solvent and other interfering species. 3) We will combine expertise gained from the above programs to design and fabricate fully functional mimics of the electron transferase family of flavoenzymes. These investigations will address key issues of recognition in water and control of enzyme mimic redox potentials, and will culminate with the creation of a functional electron shuttle designed to fully replicate the function of an electron transferase.