A LARGE MOSAIC CCD CAMERA FOR CRYO EM

用于冷冻电镜的大型马赛克 CCD 相机

• 批准号: 6766837
• 负责人: XUONG NGUYEN-HUU
• 金额: $ 78.43万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6766837
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; charge coupled device camera; computer program /software; computer system design /evaluation; cryoelectron microscopy; image processing; lens; phosphorescence; tomography

DESCRIPTION (provided by applicant): The goal of the proposed project is to design and build a large mosaic of CCD detectors to provide an improved film substitute for direct digital recording of data for biological electron rnicroscopy. Such a system will be immediately applicable and beneficial to cryo-electron microscopy (cryo-EM) of macromolecules and 3D electron tomography of macromolecular complexes, organelles, and cellular subsystems. For molecular microscopy (with cryo-EM), this system will enable the routine collection of up to l million images of large macromolecular complexes, facilitating and accelerating the determination of high-resolution structures of protein complexes, without the difficulty of growing large crystals of purified materials (as is otherwise the case with protein crystallography). The same new detector technology will also allow the collection of wide-field images for intermediate resolution 3D microscopy spanning multiple spatial scales, to enable determinations of the precise organization of supramolecular complexes throughout large functional domains of cells in tissues. Building on the recent success of lens-coupled CCD camera technology, our goal is to build a new detector containing a mosaic of 9 CCD detectors, each with its own lens that can cover an overlapping portion of a scintillator screen of 15 cm x l5 cm. This mosaic detector will have a functional resolution of 5600 x 5600 pixels. We will initially design and test a single "module" of this detector with one back-side illuminated 2K x 2K pixel (13.5 gm) CCD sensor and one prototype lens. This will be followed by the design and development of a 2 x 2 mosaic of detectors and lenses. This moderately complex mosaic camera will allow us to work out all design and development issues before making the 9 CCD mosaic in the 3rd year. A significant effort during all 3 years will be to map out all detector distortions and to develop software to seamlessly stitch together detector subimages.

描述(申请人提供)：拟议项目的目标是设计和建造一个大型的电荷耦合器件探测器，以提供一种改进的胶片替代生物电子显微镜数据的直接数字记录。这样的系统将立即适用于大分子的冷冻电子显微镜(Cryo-EM)和大分子络合物、细胞器和细胞子系统的3D电子断层扫描。对于分子显微镜(使用冷冻EM)，该系统将能够常规收集多达L百万张的大分子复合体图像，促进和加快蛋白质复合体高分辨率结构的确定，而不会像蛋白质结晶学那样难以生长纯化材料的大晶体(与蛋白质结晶学不同)。同样的新探测器技术还将允许收集跨越多个空间尺度的中分辨率3D显微镜的广域图像，从而能够确定组织中细胞的大型功能域中超分子复合体的精确组织。 基于最近成功的透镜耦合CCD相机技术，我们的目标是建造一种新的探测器，其中包含9个CCD探测器，每个探测器都有自己的透镜，可以覆盖15厘米x 15厘米的闪烁体屏幕的重叠部分。这款马赛克探测器的功能分辨率为5600 x 5600像素。我们将首先设计和测试该探测器的一个单独的“模块”，其中包括一个背面照明的2K x 2K像素(13.5克)的ccd传感器和一个原型透镜。随后将设计和开发2x2马赛克探测器和透镜。这款中等复杂的马赛克相机将允许我们在第三年制作9 CCD马赛克之前解决所有设计和开发问题。在所有三年中，一项重要的努力将是绘制出所有探测器的扭曲情况，并开发软件将探测器的子图像无缝拼接在一起。