Stress-bearing Structures In Muscle And Muscle Diseases

肌肉和肌肉疾病中的承压结构

• 批准号: 6823105
• 负责人: KUAN WANG
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6823105
• 关键词: X ray crystallography; biological signal transduction; biomechanics; cell component structure /function; cytoskeletal proteins; electron microscopy; fish; genetic disorder; intermediate filaments; magnetic resonance imaging; microfilaments; molecular assembly /self assembly; molecular cloning; muscle; muscle contraction; muscle disorders; muscle function; muscle proteins; muscle tension; myocardium; myofibrils; protein kinase; protein structure function; sarcomeres; striated muscles

The cytoplasm of striated muscle cells contains, besides actin and myosin filaments, contains at least two interconnected lattices. An intermediate filament lattice envelops and links all sarcomeres to the membrane skeleton, mitochondria, nuclei, and sarcoplasmic reticulum. Inside the sarcomere, a cytoskeletal matrix consisted of a set of elastic titin filaments and a set of inextensible nebulin filaments provides structural continuity. Both lattices generate restoring force. Active force and elastic force are transmitted through specialized anchor structures of the sarcomere. One important stress-bearing structure is the Z line, a dense and narrow structure that anchors and organizes four major filaments: actin, titin, nebulin and desmin filaments. The Z lines are now known to play important roles in the structural organization of sarcomere, the transmission of mechanical forces as well as the stress signaling pathways. Its dense structure however poses technical challenges and the variability of protein composition made it difficult to generalize findings from one muscle to the next. Our projects address the Z line structure and function from several prospectives. 1. What are the roles of titin, nebulin (skeletal muscles), nebulette (a nebulin-ike protein in the heart) in the assembly and integrity of the Z line in vertebrate muscle? 2. What are the composition and structure of the unusually broad Z line of sonic muscle of Midshipman fish? 3. What are the roles of protein kinases, nonmuscle myosin and other signaling proteins in the function of the Z lines? 4. What is its relationship to the anomalous nemaline rod Z bodies found in aging heart muscle, in diseased skeletal muscle known as nemaline myopathy? The distribution of titin, nebulin, nebulette nonmuscle myosin IIB and into the myofibrils and the Z lines are being studied with fluorescence techniques with either monoclonal antibodies to these proteins, or by the use of fluorescent fusion proteins synthesized within the muscle cells. To identify protein composition, especially the proteins that interact with titin, nebulin and nebulette in the Z line, we are applying both molecular biological methods (yeast two hybrid screening), as well as biochemical techniques to search for interacting proteins. We have succeeded in resolving the high-resolution structure of the unusually broad Z band (1 micron, roughly 20 times the wide of vertebrate Z lines) in the sonic muscle of Midshipman fish is being studied by electron microscopy, X-ray diffraction and biochemical methods. Interestingly, the Z band are also attachment sites of a very elaborate intermediate filaments lattice and junctional complexes at the Z band that provide the necessary radial force to assemble and maintain the tubular shape of the sonic muscle fiber. We are initiating the cloning of proteins involved in the structure of the Z bands and the comparison of protein and transcript profiles of nemaline myopathy muscles. To visualize the contraction of the sonic muscle, we have also succeeded in visualizing the detailed organization of muscle fibers in intact sonic muscle by magnetic resonance imaging (MRI). These studies are important in the understanding of how contractile machinery assembles during development, how it dissembles during remodeling of muscle tissues, how tension are transmitted during muscle activities and how muscles malfunction in nemaline myopathy and other muscle diseases.

横纹肌细胞的细胞质除了含有肌动蛋白和肌球蛋白外，还含有至少两个相互连接的晶格。中间纤维网格包裹所有肌节并将其连接到膜骨架、线粒体、细胞核和肌浆网。在肌节内，由一组弹性肌联蛋白细丝和一组不可伸展的星云蛋白细丝组成的细胞骨架基质提供结构连续性。两个晶格都产生恢复力。主动力和弹性力通过肌节的专门锚结构传递。一个重要的应力承载结构是Z线，这是一种密集而狭窄的结构，它锚定并组织四种主要的细丝：肌动蛋白、肌联蛋白、星云蛋白和结蛋白细丝。目前已知Z线在肌节的结构组织、机械力的传递以及应激信号通路中起重要作用。 然而，它的密集结构带来了技术挑战，蛋白质组成的可变性使得很难将研究结果从一块肌肉推广到另一块肌肉。我们的项目从几个方面解决Z线的结构和功能。1.肌联蛋白、星云蛋白（骨骼肌）、星云蛋白（心脏中的一种星云蛋白样蛋白）在脊椎动物肌肉中Z线的组装和完整性中起什么作用？2.什么是组成和结构的异常广泛的Z线声波肌肉的海军军官学校鱼？3.蛋白激酶、非肌肉肌球蛋白和其他信号蛋白在Z线功能中的作用是什么？4.它与在衰老的心肌中发现的异常杆状Z体有什么关系？在患病的骨骼肌中发现的异常杆状Z体被称为杆状肌病？肌联蛋白，nebulin，neblette非肌肌球蛋白IIB和肌原纤维和Z线的分布正在研究与荧光技术与这些蛋白质的单克隆抗体，或通过使用荧光融合蛋白内合成的肌肉细胞。为了鉴定蛋白质组成，特别是与Z线中的肌联蛋白、星云蛋白和星云蛋白相互作用的蛋白质，我们应用分子生物学方法（酵母双杂交筛选）以及生物化学技术来寻找相互作用的蛋白质。我们已经成功地解决了异常宽的Z带（1微米，大约20倍的脊椎动物Z线的宽度）的高分辨率结构的声音肌肉的海军军官学校的鱼正在研究电子显微镜，X射线衍射和生物化学方法。有趣的是，Z带也是非常精细的中间丝晶格和Z带处的连接复合物的附着位点，其提供必要的径向力以组装和维持声波肌纤维的管状形状。我们正在开始克隆与Z带结构有关的蛋白质，并比较线虫肌病肌肉的蛋白质和转录谱。为了可视化发声肌的收缩，我们还通过磁共振成像（MRI）成功地可视化了完整发声肌中肌纤维的详细组织。这些研究对于了解收缩机制在发育过程中如何组装，在肌肉组织重塑过程中如何分解，肌肉活动过程中张力如何传递以及线状体肌病和其他肌肉疾病中肌肉如何发生功能障碍非常重要。