Adenosine and modulation of synaptic transmission

腺苷和突触传递的调节

• 批准号: 6723733
• 负责人: SUSAN A MASINO
• 金额: $ 28.61万
• 依托单位: TRINITY COLLEGE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6723733
• 关键词: acidity /alkalinity; adenosine; calcium flux; cerebral ischemia /hypoxia; electrophysiology; gene targeting; hippocampus; intracellular transport; laboratory mouse; laboratory rat; neural transmission; neurotransmitter transport; purinergic receptor; tissue /cell culture

DESCRIPTION(From applicant's abstract): In his long-standing interests in determining neurobiological mechanisms of adenosine, the principal investigator has constructed a novel hypothesis that release of intracellular adenosine results not only from an increase in intracellular calcium levels but also from an acidification of intracellular milieu, as measured by an decrease in intracellular pH values. This proposal will predominately use the hippocampal slice preparation to explore various aspects of adenosine neurobiology from an electrophysiological point of view. The first aim will explore the role of intracellular pH and calcium in mediating adenosine release. These studies will explore whether changes in intracellular calcium and pH are both necessary and sufficient to produce adenosine release by using a variety of reagents which affect the intracellular milieu. The second specific aim explore whether adenosine plays an important role in regulating the basic excitability of neurons by performing experiments in adenosine A1 receptor-knockout mice. These studies will determine whether hippocampal slices from KO mice are hyperexcitable, and whether KO of adenosine A1 receptors affect the function of other inhibitory receptors.

描述（来自申请人摘要）： 他长期致力于确定神经生物学机制， 腺苷，首席研究员构建了一个新的假设， 细胞内腺苷的释放不仅是由于 细胞内钙离子水平，而且从细胞内的酸化 细胞内pH值的降低，如通过细胞内pH值的降低所测量的。这项建议 将主要使用海马切片制备来探索各种 腺苷神经生物学方面从电生理学的观点。 第一个目标将探讨细胞内pH值和钙离子的作用， 介导腺苷释放。这些研究将探讨是否存在变化 细胞内钙和pH都是必需的，并且足以产生 腺苷释放通过使用各种试剂，影响细胞内 环境。第二个具体目标是探索腺苷是否在 在调节神经元基本兴奋性方面的作用 腺苷A1受体敲除小鼠中。这些研究将确定是否 KO小鼠的海马切片是过度兴奋的， A1受体影响其他抑制性受体的功能。

项目成果

Effects of adenosine and cadmium on presynaptic fiber spikes in the CA1 region of rat hippocampus in vitro.

腺苷和镉对体外大鼠海马 CA1 区突触前纤维尖峰的影响。

• DOI: 10.1016/0028-3908(93)90071-a
• 发表时间: 1993
• 期刊: Neuropharmacology
• 影响因子: 4.7
• 作者: Dunwiddie,TV;Miller,KK
• 通讯作者: Miller,KK

A transient increase in temperature induces persistent potentiation of synaptic transmission in rat hippocampal slices.

温度的短暂升高会诱导大鼠海马切片中突触传递的持续增强。

• DOI: 10.1016/s0306-4522(00)00431-0
• 发表时间: 2000
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: Masino,SA;Dunwiddie,TV
• 通讯作者: Dunwiddie,TV

Bidirectional synaptic plasticity in the dentate gyrus of the awake freely behaving mouse.

清醒自由行为小鼠齿状回的双向突触可塑性。

• DOI: 10.1016/j.jneumeth.2007.08.001
• 发表时间: 2008
• 期刊: Journal of neuroscience methods
• 影响因子: 3
• 作者: Koranda,JessicaL;Masino,SusanA;Blaise,JHarry
• 通讯作者: Blaise,JHarry

Extracellular adenosine concentrations in hippocampal brain slices and the tonic inhibitory modulation of evoked excitatory responses.

• 发表时间: 1994-02
• 期刊: The Journal of pharmacology and experimental therapeutics
• 作者: T. Dunwiddie;Liping Diao

Adenosine, ketogenic diet and epilepsy: the emerging therapeutic relationship between metabolism and brain activity.

• DOI: 10.2174/157015909789152164
• 发表时间: 2009-09
• 期刊: Current neuropharmacology
• 影响因子: 5.3
• 作者: Masino SA;Kawamura M;Wasser CD;Pomeroy LT;Ruskin DN
• 通讯作者: Ruskin DN