Robust DNA Biochip Surfaces

坚固的 DNA 生物芯片表面

• 批准号: 6736479
• 负责人: TODD C. STROTHER
• 金额: $ 17.52万
• 依托单位: GENTEL BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-03 至 2005-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6736479
• 关键词: biomaterial development /preparation; biomaterial evaluation; biotechnology; carbon; chemical synthesis; genotype; heat; microarray technology; nucleic acid chemical synthesis; nucleic acid hybridization; nucleic acid sequence; surface coating; surface property; thermostability

DESCRIPTION (provided by applicant): Since microarray biochips were invented, they have become instrumental in gene expression, high throughput screening, and drug discovery. Current glass-based biochips, however, are chemically unstable and are unable to withstand applications requiring multiple cycles, high temperature, or high pH. Applications requiring these challenging conditions, such as on-chip parallel DNA sequencing have been unable to benefit from the advantages of biochips, because the inherent limitations of the current substrates and surface chemistry. GenTel scientists have developed proprietary chemistry that uses the UV mediated attachment of alkenes to glassy carbon and diamond surfaces. This innovative chemistry will expand the applicability of biochips to these demanding applications. Diamond and carbon substrates can be obtained surprisingly cheaply and possess strong C-C bonds that are exceptionally rugged, both chemically and physically. The successful application of these novel substrates and surface chemistry to a model on-chip sequencing system will demonstrate their ability to be used in areas previously inaccessible by currently available biochips. The specific goal of the Phase I proposal is to establish the technical merit and feasibility of creating more durable and robust carbon substrates that can withstand extreme thermal or chemical conditions for DNA microarray applications. A following Phase II project will develop prototypes of these surfaces to researchers interested in on-chip parallel DNA sequencing, genotyping, and biosynthesis.

描述（由申请人提供）：自从微阵列生物芯片被发明以来，它们已经成为基因表达、高通量筛选和药物发现的工具。然而，目前的玻璃基生物芯片是化学不稳定的，不能承受需要多个周期，高温，或高pH值的应用。应用程序需要这些具有挑战性的条件，如芯片上的并行DNA测序已经无法受益于生物芯片的优点，因为目前的基板和表面化学的固有限制。 GenTel的科学家们已经开发出专有的化学物质，使用紫外线介导的烯烃附着在玻璃碳和金刚石表面。这种创新的化学将扩大生物芯片的适用性，这些要求苛刻的应用。 金刚石和碳基质可以令人惊讶地便宜地获得，并且具有强的C-C键，其在化学和物理上都非常坚固。这些新的基板和表面化学的成功应用到一个模型芯片上的测序系统，将证明他们的能力，用于以前无法进入的地区目前可用的生物芯片。 第一阶段提案的具体目标是建立技术优点和可行性，以创建更耐用和更坚固的碳基底，可以承受极端的热或化学条件的DNA微阵列应用。接下来的第二阶段项目将为对芯片上并行DNA测序、基因分型和生物合成感兴趣的研究人员开发这些表面的原型。