Rapid and Inexpensive Biochip-Based Allergy Testing

快速且廉价的基于生物芯片的过敏测试

• 批准号: 6791505
• 负责人: TODD C. STROTHER
• 金额: $ 16.73万
• 依托单位: GENTEL BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-15 至 2005-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6791505
• 关键词: amines; biochemistry; bioengineering /biomedical engineering; biomedical equipment development; clinical biomedical equipment; clinical research; diagnosis design /evaluation; enzyme linked immunosorbent assay; human subject; human tissue; hypersensitivity; immunoglobulin E; isothiocyanates; microarray technology; molecular film; peptides; polyethylene glycols; polymers; rapid diagnosis; serology /serodiagnosis; succinimides; surface property

GenTel BioSurfaces (GenTel) seeks to establish the technical merit and feasibility of an allergen biochip for use as a rapid, inexpensive and easy-to-use in vitro method for identifying elevated allergen-specific IgE titers in serum. This biochip is based upon a uniform gold surface that is highly amenable to chemical modification and uses a unique microchannel technology (Array 2 Microchannels). On-chip microchannels enable many discreet interactions to be studied simultaneously and allow rapid, on-chip generation of a standard curve. Quantification is obtained in an analogous manner to an enzyme linked immunosorbent assays (ELISAs). Together, these technologies allow GenTel to develop a robust allergen biochip that can be used to rapidly quantitate allergen-specific IgE titers in serum. This biochip will find applications in the clinical and research communities including, but not limited to, allergen-specific IgE profiling of human patients, monitoring vaccine and therapeutic antibody efficacy in animal models, antigenic determination and localization of antibody populations. The objectives of this proposal are to: (1) Select optimal surface chemistry for attachment of allergens as measure by the highest, specific binding of target IgE; (2) develop and determine optimal surface chemistry for blocking non-specific adsorption of human serum components to the array surface as measured by the highest signal: noise ratio and (3) to validate the ability of the proposed allergen biochip to quantitatively determine the titer of an allergen-specific IgE using our innovative Array" Microchannels by comparing the results to those obtained by ELISA. A follow-up Phase II proposal will broaden the number of allergens tested and further develop our Array 2 Microfluidics technology by integrating sample purification with the biochip. This will enable direct application of whole blood to the biochip and an inexpensive chip-reading allergy test with a turnaround time of about one hour.

GenTel BioSurfaces（GenTel）旨在建立过敏原生物芯片的技术优点和可行性，以作为一种快速，廉价和易于使用的体外方法，用于识别血清中过敏原特异性IgE滴度升高。这种生物芯片基于均匀的金表面，非常适合化学修饰，并使用独特的微通道技术（阵列2微通道）。芯片上的微通道可以同时研究许多离散的相互作用，并可以快速在芯片上生成标准曲线。以与酶联免疫吸附测定（ELISA）类似的方式获得定量。这些技术使GenTel能够开发出一种强大的过敏原生物芯片，可用于快速定量血清中过敏原特异性IgE滴度。这种生物芯片将在临床和研究领域得到应用，包括但不限于过敏原特异性IgE 分析人类患者、监测动物模型中的疫苗和治疗性抗体功效、抗原测定和抗体群体的定位。 本提案的目标是：（1）选择最佳表面化学物质，用于附着过敏原，通过目标IgE的最高特异性结合来衡量;（2）开发并确定最佳表面化学物质，用于阻止人血清成分非特异性吸附到阵列表面，通过最高信号来衡量：噪声比和（3）验证所提出的过敏原生物芯片定量测定过敏原特异性IgE滴度的能力， 我们的创新阵列”微通道通过比较的结果，通过ELISA获得。 后续的第二阶段提案将扩大测试的过敏原数量，并通过将样品纯化与生物芯片相结合来进一步开发我们的Array 2微流体技术。这将使全血直接应用于生物芯片和一个便宜的芯片读取过敏试验，周转时间约为一小时。