Gene expression profiles in lung cancer using cDNA array

cDNA阵列是一种有效的方法，可以快速分析在各种感兴趣的组织中差异表达的许多基因。使用基于Affyphine寡核苷酸的cDNA阵列，我们迄今已调查了65个原发性组织，并检查了肿瘤和正常肺组织之间的基因表达差异。在肺癌中受影响的分子途径中，我们观察到参与TGF-β信号传导的基因的一致下调。我们进一步观察到SMAD 6和SMAD 7的丢失似乎与NSCLC患者中肿瘤相关生存期的改善相关。使用siRNA技术的体外实验目前正在进行中，以检查肺肿瘤中SMAD 6失活的机制。我们还使用成熟的TGF-β功能测定来检查肺肿瘤和正常细胞之间的功能差异。

cDNA array is a powerful method that allows for rapid analysis of many genes differentially expressed in a variety of tissues of interest. Using Affymetrix oligo-nucleotide based cDNA arrays, we have so far surveyed 65 primary tissues and examined gene expression differences between the tumor and normal lung tissues. Among the molecular pathways affected in lung cancers, we observed a consistent down regulation of genes involved in the TGF-beta signaling. We further observed that the loss of SMAD6 and SMAD7 appeared to be correlated with an improved tumor-related survival in NSCLC patients. In vitro experiments using siRNA technology are currently underway to examine the mechanism of SMAD6 inactivation in lung tumors. We are also using well established assays of TGF-beta functions to examine their functional differences between the lung tumor and normal cells.