Molecular basis of PGP9.5 overexpression in lung cancer
Molecular basis of PGP9.5 overexpression in lung cancer
批准号:
6755662
负责人:
Jin Jen
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
biological models biomarker carcinogenesis differentiation antigens gene expression genes guanine nucleotide binding protein laboratory mouse ligase lung lung neoplasms model design /development molecular genetics neoplasm /cancer genetics neoplastic process protein protein interaction site directed mutagenesis yeast two hybrid system
中文摘要
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英文摘要
We have previously identified that PGP9.5 is frequently overexpressed in lung cancers. We have also used yeast two-hybrid assay to identify genes that interact with PGP9.5. We recently showed that three genes, JAB1, UBC9 and RanBPM, are associated with PGP9.5 both in vitro and in vivo. Site-directed mutagenesis method is being used to determine the regions of PGP9.5 protein that interact with these factors. We are also also in the process of developing a mouse model that allows for tissue specific expression of PGP.5 in the small airway of the lung to determine the role of PGP9.5 in lung tumorigenesis.
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