DEVELOPMENT OF A FLUORESCENCE-BASED BIOFLUID VISCOMETER

描述(申请人提供)：到目前为止，流体粘度通过以下方式获得 机械手段。流体被剪切，抗剪切阻力是 与粘度有关。所有的机械方法都有一个共同点，即 测量过程非常耗时，并且需要相对大量的 流体。测量仪器需要严格清洁，这增加了 测量系列的时间要求。当测量生物流体时， 含有大量蛋白质和/或胶体，蛋白质沉积在 仪器表面会引入误差，就像 液/气界面。荧光分子旋转器，变化的分子 它们的荧光量子产率与环境的粘度有关，允许 在没有上述缺点的生物流体中进行粘度测量。一个 粘度计是在这项开发拨款中提出的，它允许粘度 基于荧光分子转子的生物体液测量。第一 这一阶段的赠款涉及特定的问题，如转子蛋白 各种胶体的相互作用、可测量性、流体浊度和 吸收。它将试图找到特定的分子和特定的 一种测量技术，可以精确地测量粘度 超过标准粘度计。在第二阶段，有两个原型 将开发一系列基于荧光的粘度计。志存高远 精确度适用于实验室使用，第二种将是快速、低量 电池供电的现场可用设备。这两款设备都将在 涉及血浆、淋巴液和间质的研究领域 流体粘度。另一个应用是监测血液 创伤医学中使用高粘度血浆扩张器的替代。

DESCRIPTION (provided by applicant): To date, fluid viscosity is obtained by mechanical means. Fluids are sheared, and the resistance against shear is related to viscosity. All mechanical methods have in common that the measurement process is time-consuming and requires relatively large amounts of fluid. The measurement apparatus needs scrupulous cleaning, which increases time requirements for measurement series. When measuring biofluids, which contain large amounts of proteins and/or colloids, protein deposition at the instrument surface introduces errors, as does the protein interaction at the liquid/air interface. Fluorescent molecular rotors, molecules that change their fluorescence quantum yield with the viscosity of the environment, allow viscosity measurements in biofluids without the above disadvantages. A viscometer is proposed in this development grant that allows viscosity measurements of biofluids based on fluorescent molecular rotors. The first phase of this grant addresses specific issues such as rotor-protein interaction, measurability of various colloids, fluid turbidity and absorption. It will be attempted to find a specific molecule and a specific measurement technique that allows viscosity measurements with a precision that exceeds that of standard viscometers. During the second phase, two prototypes of fluorescence-based viscometers will be developed. One aims at high accuracy for laboratory usage, and the second will be a fast, low-volume battery-powered field-usable device. Both devices will have applications in research areas that involve blood plasma, lymphatic fluid and interstitial fluid viscosity. One additional application is the monitoring of blood replacement using high-viscosity plasma expanders in trauma medicine.