DEVELOPMENT OF A FLUORESCENCE-BASED BIOFLUID VISCOMETER

描述（由申请人提供）：迄今为止，流体粘度通过以下方法获得： 机械手段。流体被剪切，并且抗剪切的阻力是 与粘度有关。所有的机械方法都有一个共同点， 测量过程是耗时的，并且需要相对大量的 液测量设备需要仔细清洁，这增加了 测量系列的时间要求。当测量生物流体时， 含有大量的蛋白质和/或胶体，蛋白质沉积在 仪器表面引入误差，蛋白质相互作用也是如此。 液体/空气界面。荧光分子转子，改变的分子 它们的荧光量子产率与环境的粘度有关， 生物流体中的粘度测量而没有上述缺点。一 粘度计建议在这个发展赠款，使粘度 基于荧光分子转子的生物流体测量。第一 这一阶段的赠款解决具体问题，如转子蛋白质 各种胶体的相互作用、可测量性、流体浊度和 吸收将试图找到一种特定的分子和一种特定的 测量技术，允许粘度测量的精度， 超过了标准粘度计。在第二阶段，两个原型 荧光粘度计将被开发。一个目标高 第二个将是快速，低容量的 电池供电的现场可用设备。这两款设备都将在 研究领域涉及血浆、淋巴液和间质 流体粘度另一个应用是血液监测 在创伤医学中使用高粘度血浆膨胀剂替代。

DESCRIPTION (provided by applicant): To date, fluid viscosity is obtained by mechanical means. Fluids are sheared, and the resistance against shear is related to viscosity. All mechanical methods have in common that the measurement process is time-consuming and requires relatively large amounts of fluid. The measurement apparatus needs scrupulous cleaning, which increases time requirements for measurement series. When measuring biofluids, which contain large amounts of proteins and/or colloids, protein deposition at the instrument surface introduces errors, as does the protein interaction at the liquid/air interface. Fluorescent molecular rotors, molecules that change their fluorescence quantum yield with the viscosity of the environment, allow viscosity measurements in biofluids without the above disadvantages. A viscometer is proposed in this development grant that allows viscosity measurements of biofluids based on fluorescent molecular rotors. The first phase of this grant addresses specific issues such as rotor-protein interaction, measurability of various colloids, fluid turbidity and absorption. It will be attempted to find a specific molecule and a specific measurement technique that allows viscosity measurements with a precision that exceeds that of standard viscometers. During the second phase, two prototypes of fluorescence-based viscometers will be developed. One aims at high accuracy for laboratory usage, and the second will be a fast, low-volume battery-powered field-usable device. Both devices will have applications in research areas that involve blood plasma, lymphatic fluid and interstitial fluid viscosity. One additional application is the monitoring of blood replacement using high-viscosity plasma expanders in trauma medicine.