Noninvasive imaging of tissue-engineered blood vessels

组织工程血管的无创成像

• 批准号: 7035994
• 负责人: MARK Andreas HAIDEKKER
• 金额: $ 19.69万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7035994
• 关键词: angiography; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomechanics; biomedical equipment development; bioreactors; blood vessel transplantation; image processing; optical tomography; tissue engineering

DESCRIPTION (provided by applicant): In this project we will develop novel imaging technology for quality control of tissue-engineered blood vessels (TEBV). Recent developments in the field of tissue engineering led to the development of completely biological living vascular graft that solves major problems associated with synthetic materials such as inflammation and infection. Since this type of vascular graft is not produced in large batches, but rather on a patient-to-patient basis, close monitoring of the development process is paramount. Unlike synthetic grafts, mechanical testing of duplicate grafts is very costly and not very reliable. Hence, there is growing demand for noninvasive methods to predict biomechanical properties. However, due to the novelty of the manufacturing process, no suitable imaging modalities exist. The purpose of this project is to develop and refine methods based on transillumination optical tomography (OT) and optical coherence tomography (OCT) for thin tissue samples (vascular grafts are 300 - 500 Lim thick), and relate the images to biomechanical properties. In this project, the OT and OCT scanners will be developed. This includes the acquisition control and the development of image reconstruction and enhancement algorithms. The goal is to achieve optimum signal-to-noise ratio at a resolution of 10 pm and short (5-20 minute) scanning times. Specialized bioreactors will be developed that allow to obtain images of the growing vessels inside the bioreactor without breaking sterility, allowing for routine examination. We will determine the capabilities of the new modalities in comparison with histology and with respect to biomechanical testing. The proposed OT and OCT imaging techniques are not limited to TEBV. Additional applications include conventional grafts as well as other tissue engineering products, such as artificial skin and cartilage. The results of this project will provide novel imaging methods that will accelerate the development and clinical availability of tissue engineering products.

描述(申请人提供)：在这个项目中，我们将为组织工程血管(TEBV)的质量控制开发新的成像技术。组织工程领域的最新发展导致了完全生物的活体血管移植物的发展，它解决了与合成材料相关的主要问题，如炎症和感染。由于这种类型的血管移植物不是大批量生产的，而是在患者对患者的基础上生产的，因此密切监测开发过程是至关重要的。与合成移植物不同，重复移植物的机械测试非常昂贵，而且不太可靠。因此，对预测生物力学特性的非侵入性方法的需求越来越大。然而，由于制造工艺的新颖性，没有合适的成像方式存在。本项目的目的是发展和改进基于透光光学层析成像(OT)和光学相干层析成像(OCT)的薄组织样本(血管移植物厚度为300-500 LIM)的方法，并将图像与生物力学特性相关联。在这个项目中，将开发OT和OCT扫描仪。这包括采集控制以及图像重建和增强算法的开发。其目标是在分辨率为10 PM且扫描时间较短(5-20分钟)的情况下实现最佳信噪比。将开发专门的生物反应器，以便在不破坏无菌的情况下获得生物反应器内不断生长的血管的图像，从而进行常规检查。我们将通过与组织学和生物力学测试的比较来确定新模式的能力。建议的OT和OCT成像技术并不局限于TEBV。其他应用包括传统的移植物以及其他组织工程产品，如人造皮肤和软骨。该项目的成果将提供新的成像方法，加速组织工程产品的开发和临床应用。