Transplantation of MAPC-derived hepatocyte-like cells

描述（由申请人提供）：我们的实验室已经从人类和啮齿动物骨髓或其他组织中产生的人间充质干细胞培养物中分离出多能成人祖细胞或MAPC，或罕见细胞。MAPCs能够在体外分化为三种胚层细胞。此外，MAPC在体外分化为具有肝细胞形态、表型和功能特征的细胞。我们假设未分化的MAPC或诱导分化为肝细胞样分化细胞的MAPC移植将植入天然肝脏并有助于功能再生。使用逆转录酶-部分肝切除术模型，我们将评估MAPC和肝细胞样分化MAPC是否能够移植到天然肝脏中。将使用FISH， RT-PCR，细胞发光和直接肝质量测量来评估移植。移植细胞与原生细胞的融合将通过Cre/Lox重组的方法进行评估。

DESCRIPTION (provided by applicant): Our lab has isolated multipotent adult progenitor cells, or MAPC, rare cells within human mesenchymal stem cell cultures generated from human and rodent bone marrow or other tissues. MAPCs are capable of differentiating in vitro to cells of the three germ layers. In addition, MAPC differentiate into cells with morphological, phenotypic, and functional characteristics of hepatocytes in vitro. We hypothesize that transplantation of undifferentiated MAPC or MAPC induced to differentiate to hepatocyte-like differentiated cells will engraft into the native liver and contribute to functional regeneration. Using a retrorsine - partial hepatectomy model we will evaluate whether MAPC and hepatocyte-like differentiated MAPCs are able to engraft into native liver. Evaluation of engraftment will be done using FISH, RT-PCR, cell luminescence, and direct liver mass measurements. Fusion of transplanted cells to native cells will be evaluated with a method involving Cre/Lox recombination.