Transplantation of MAPC-derived hepatocyte-like cells

MAPC 来源的肝细胞样细胞移植

• 批准号: 6935748
• 负责人: NATHANIEL S KREYKES
• 金额: $ 6.19万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-12 至 2008-09-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935748
• 关键词: fluorescent in situ hybridization; laboratory mouse; liver cells; liver regeneration; pluripotent stem cells; polymerase chain reaction; postdoctoral investigator; stem cell transplantation

DESCRIPTION (provided by applicant): Our lab has isolated multipotent adult progenitor cells, or MAPC, rare cells within human mesenchymal stem cell cultures generated from human and rodent bone marrow or other tissues. MAPCs are capable of differentiating in vitro to cells of the three germ layers. In addition, MAPC differentiate into cells with morphological, phenotypic, and functional characteristics of hepatocytes in vitro. We hypothesize that transplantation of undifferentiated MAPC or MAPC induced to differentiate to hepatocyte-like differentiated cells will engraft into the native liver and contribute to functional regeneration. Using a retrorsine - partial hepatectomy model we will evaluate whether MAPC and hepatocyte-like differentiated MAPCs are able to engraft into native liver. Evaluation of engraftment will be done using FISH, RT-PCR, cell luminescence, and direct liver mass measurements. Fusion of transplanted cells to native cells will be evaluated with a method involving Cre/Lox recombination.

描述(申请人提供)：我们的实验室已经分离出多能成体祖细胞，或MAPC，从人和啮齿动物的骨髓或其他组织中产生的人间充质干细胞培养中的稀有细胞。MAPC能够在体外分化为三种胚层的细胞。此外，MAPC在体外可分化为具有肝细胞形态、表型和功能特征的细胞。我们推测，未分化的MAPC或MAPC诱导分化为肝细胞样分化细胞的移植将植入天然肝脏，并有助于功能再生。利用逆转录-部分肝切除模型，我们将评估MAPC和肝细胞样分化的MAPC是否能够植入自体肝脏。植入的评估将使用FISH、RT-PCR、细胞发光和直接肝质量测量进行。移植细胞与自然细胞的融合将通过一种涉及Cre/Lox重组的方法进行评估。