Building the retinal mosaic for color vision in flies

构建果蝇色觉的视网膜马赛克

• 批准号: 6935520
• 负责人: PREETMONINDER LIDDER
• 金额: $ 4.21万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935520
• 关键词: Drosophilidae; arthropod genetics; cell differentiation; color visions; developmental genetics; developmental neurobiology; gene expression; genetic mapping; genetic promoter element; genetic regulatory element; messenger RNA; microarray technology; mutant; neurogenesis; nucleic acid sequence; phenotype; postdoctoral investigator; protein structure function; pupil; retina; rhodopsin; tissue mosaicism; transcription factor; visual photoreceptor

DESCRIPTION (provided by applicant): Color vision requires the comparison of the output of classes of photoreceptor cells (PRs) that respond differently to the wavelength of incoming light. In Drosophila, the primary visual system is composed of pale (p) and yellow (y) ommatidia, which are distributed in a stochastic manner with a conserved ratio of 30% (p) to 70% (y). Recent evidence has suggested that the gene spineless (ss), is responsible for the formation of the ommatidial mosaic by defining the y ommatidia. The studies described in this proposal are aimed at testing the hypothesis that the stochastic expression of spineless in a subset of PRs is the primary event that leads to the creation of the complex retinal mosaic used for color vision. To test this hypothesis, we will address three specific aims: 1) functional analysis of spineless, 2) determination of factors controlling the stochastic expression of ss, and 3) identification of new molecular phenotypes of ss. These experiments will be performed using a variety of molecular, genetic and genomic approaches. The successful completion of this work should provide a wealth of information about the basic mechanisms involved in y/p PR subtype specification. Since the mechanisms of eye development and differentiation are conserved from flies to humans, ultimately, a more detailed knowledge of the fly retinal mosaic will lead to a better understanding of the vertebrate retina.

描述（由申请人提供）：色觉需要比较对入射光波长有不同反应的感光细胞（PR）的输出。在果蝇中，初级视觉系统由浅色（p）和黄色（y）小眼组成，它们以随机方式分布，保守率为30%（p）至70%（y）。最近的证据表明，基因spineless（ss），是通过定义yommatidia形成小眼镶嵌。本提案中描述的研究旨在检验以下假设：PRs子集中无脊椎的随机表达是导致创建用于色觉的复杂视网膜马赛克的主要事件。为了验证这一假设，我们将解决三个具体的目标：1）无脊椎的功能分析，2）确定控制SS的随机表达的因素，和3）新的SS分子表型的鉴定。这些实验将使用各种分子、遗传和基因组方法进行。这项工作的成功完成，应提供丰富的信息的基本机制参与y/p PR亚型规范。由于眼睛发育和分化的机制是保守的从苍蝇到人类，最终，更详细的知识苍蝇视网膜马赛克将导致更好地了解脊椎动物视网膜。