Building the retinal mosaic for color vision in flies

构建果蝇色觉的视网膜马赛克

• 批准号: 7068074
• 负责人: PREETMONINDER LIDDER
• 金额: $ 4.6万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7068074
• 关键词: Drosophilidae; arthropod genetics; cell differentiation; color visions; developmental genetics; developmental neurobiology; gene expression; genetic mapping; genetic promoter element; genetic regulatory element; messenger RNA; microarray technology; mutant; neurogenesis; nucleic acid sequence; phenotype; postdoctoral investigator; protein structure function; pupil; retina; rhodopsin; tissue mosaicism; transcription factor; visual photoreceptor

DESCRIPTION (provided by applicant): Color vision requires the comparison of the output of classes of photoreceptor cells (PRs) that respond differently to the wavelength of incoming light. In Drosophila, the primary visual system is composed of pale (p) and yellow (y) ommatidia, which are distributed in a stochastic manner with a conserved ratio of 30% (p) to 70% (y). Recent evidence has suggested that the gene spineless (ss), is responsible for the formation of the ommatidial mosaic by defining the y ommatidia. The studies described in this proposal are aimed at testing the hypothesis that the stochastic expression of spineless in a subset of PRs is the primary event that leads to the creation of the complex retinal mosaic used for color vision. To test this hypothesis, we will address three specific aims: 1) functional analysis of spineless, 2) determination of factors controlling the stochastic expression of ss, and 3) identification of new molecular phenotypes of ss. These experiments will be performed using a variety of molecular, genetic and genomic approaches. The successful completion of this work should provide a wealth of information about the basic mechanisms involved in y/p PR subtype specification. Since the mechanisms of eye development and differentiation are conserved from flies to humans, ultimately, a more detailed knowledge of the fly retinal mosaic will lead to a better understanding of the vertebrate retina.

描述(申请人提供)：颜色视觉需要比较不同类别的感光细胞(PR)的输出，这些细胞对入射光的波长做出不同的反应。果蝇的初级视觉系统由浅色(P)和黄色(Y)小眼组成，它们以随机方式分布，保守率为30%(P)到70%(Y)。最近的证据表明，无刺基因(Ss)，通过定义y小眼，导致了小眼马赛克的形成。这项建议中描述的研究旨在检验一种假设，即在PR的子集中随机表达无刺是导致用于色觉的复杂视网膜马赛克的主要事件。为了验证这一假设，我们将解决三个具体的目标：1)无刺的功能分析，2)控制ss随机表达的因素的确定，以及3)ss新的分子表型的鉴定。这些实验将使用各种分子、遗传和基因组方法进行。这项工作的成功完成应该会提供关于y/p PR亚型规范所涉及的基本机制的丰富信息。由于眼睛发育和分化的机制从苍蝇到人类都是保守的，最终，对苍蝇视网膜马赛克的更详细了解将有助于更好地了解脊椎动物的视网膜。