Label-free electrical detection of proteins

蛋白质的无标记电检测

• 批准号: 6897256
• 负责人: Raj Chakrabarti
• 金额: $ 2.5万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-25 至 2005-12-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6897256
• 关键词: adsorption; bioengineering /biomedical engineering; biomedical equipment development; biosensor device; biotechnology; catalyst; electrical conductance; electrical measurement; electrical potential; electrical property; glycerol; high throughput technology; macromolecule; nanotechnology; postdoctoral investigator; protein quantitation /detection; proteomics; subtilisins

DESCRIPTION (provided by applicant): I propose herein a method by which proteins may be detected and quantitated in a label-free fashion, i.e. without the need for reporter molecules. The method is based upon field-effect detection - the electrical monitoring of molecular adsorption through the measurement of the change in surface potential that results from binding of charged biomolecules to complementary sites on a sensor surface. Previously, field effect sensors have been used to detect DNA. However, detection of proteins by this method has not yet been achieved, since the size of the surface-bound receptors prevents the target proteins from approaching the sensor sufficiently closely. My proposed solution is based on the recent discovery that the organic solvent glycerol can serve as a substitute for water as a suitable medium for a variety of proteins. I propose to exploit the lower dielectric constant of glycerol compared to water's to increase the characteristic length of detection to an extent that protein sensing becomes possible. I will initially test this approach by fabricating field effect sensors for the model protein subtilisin in glycerol. If successful, l will develop arrays of such sensors for the purpose of high throughput parallel detection, and examine the generality of my results for a wide range of proteins.

描述（由申请人提供）：我在此提出了一种可以以无标签方式检测和定量蛋白质的方法，即不需要记者分子。该方法基于现场效应检测 - 通过测量表面电势的变化对分子吸附的电气监测，这是由于带电的生物分子与传感器表面上互补位点结合而产生的。以前，现场效应传感器已用于检测DNA。但是，由于表面结合受体的大小可防止靶蛋白足够接近传感器，因此尚未实现通过这种方法检测蛋白质。我提出的解决方案是基于最近发现的，即有机溶剂甘油可以用作水作为各种蛋白质的合适培养基。我建议与水相比，利用甘油的较低介电常数，以增加检测的特征长度，以至于蛋白质感应的程度可能是可能的。最初，我将通过在甘油中为模型蛋白质枯草蛋白的现场效应传感器制造现场效应传感器来测试这种方法。如果成功的话，L将开发此类传感器的阵列，以实现高吞吐量平行检测的目的，并检查我的结果的一般性。