Analysis of MAP kinase cascades in S. cerevisiae

酿酒酵母中 MAP 激酶级联的分析

• 批准号: 6871356
• 负责人: PATRICK J WESTFALL
• 金额: $ 4.99万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6871356
• 关键词: Saccharomyces cerevisiae; biological signal transduction; cell growth regulation; environmental adaptation; environmental stressor; enzyme activity; fungal genetics; gene expression; genetic regulation; histogenesis; microarray technology; mitogen activated protein kinase; nitrogen; osmotic pressure; phosphorylation; postdoctoral investigator; site directed mutagenesis

DESCRIPTION (provided by applicant): Cells often respond to environmental cues by utilizing mitogen-activated protein (MAP) kinase cascades to regulate the expression of genes responsible for the appropriate cellular response. The experiments that I am proposing to conduct seek to advance the understanding of how cells sense environmental stress, activate the appropriate MAP kinase cascade, and then deactivate the cascade once the cell has responded. Nitrogen starvation induces the formation of pseudohyphae in S. cerevisiae via a MAP kinase cascade that shares components with a MAP kinase cascade triggered by high extracellular osmolarity. Studies from the Herskowitz lab have identified Sho1p as a potential sensor for nitrogen starvation in addition to its established role as a sensor of osmolarity. I will carry out genetic screens to identify other proteins involved in sensing low nitrogen. In addition, I shall investigate how Sho1p signaling is regulated to prevent constitutive activation of the high osmolarity glycerol and pseudohyphal growth pathways. Finally, using chemically inhibitable versions of the MAPK Hog1p in conjunction with DNA microarrays, I will identify genes regulated by Hog1p during both stress and non-stress conditions.

描述（由申请人提供）：细胞通常通过利用促分裂原活化蛋白（MAP）激酶级联来调节负责适当细胞反应的基因的表达，从而对环境信号作出反应。我建议进行的实验旨在进一步了解细胞如何感知环境压力，激活适当的MAP激酶级联反应，然后在细胞做出反应后使级联反应失活。氮饥饿诱导假菌丝的形成。通过MAP激酶级联，该级联与由高细胞外渗透压触发的MAP激酶级联共享组分。Herskowitz实验室的研究已经确定Sho1p除了作为渗透压传感器的既定作用外，还可以作为氮饥饿的潜在传感器。我将进行基因筛选，以确定其他参与感知低氮的蛋白质。此外，我将研究如何Sho1p信号调节，以防止组成性激活的高渗透压甘油和假菌丝生长途径。最后，使用化学标记的MAPK Hog1p的版本结合DNA微阵列，我将确定Hog1p在应激和非应激条件下调控的基因。