In Vitro Robotic Assay for Estrogenic Activity

雌激素活性的体外机器人测定

• 批准号: 6895492
• 负责人: Cathy Z Yang
• 金额: $ 65.33万
• 依托单位: CERTICHEM, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6895492
• 关键词: MCF7 cell; antioxidants; biotechnology; cell proliferation; computer data analysis; diagnostic tests; endocrine disorder diagnosis; estrogens; food; high performance liquid chromatography; high throughput technology; neoplastic cell culture for noncancer research; nucleic acid quantitation /detection; plastics; robotics; technology /technique development

Recent scientific investigations have shown that many chemicals used in plastics, pharmaceuticals, )esticides, cosmetics, food additives, etc. are endocrine disruptors (EDs). EDs interfere in various ways with hormonal activity to have significant adverse effects on many physiological processes at very low (picomolar to nanomolar) concentrations, especially on fetal or developing mammals. The prevalence and actions of EDs in our environment warrants the development of highly specific, sensitive, reliable, rapid, and cost-effective detection. Consequently, various governmental bodies (EPA, FDA, and ICCVAM) and proactive corporations explicitly desire an in vitro robotic assay for EA, but no such assay is commercially available, much less validated. CertiChem (CCi) has just successfully completed a Phase I SBIR project to demonstrate that it is feasible to develop a robotic assay for EA using MCF-7 cell proliferation. In this Phase II application, CCi proposes to improve and extensively validate this assay so that it can be fully commercialized in Phase III. CCi also proposes to examine the relationship between theoretical predictions of EA for a set of antioxidants used in foodstuffs and plastics and the actual EA as measured by this robotic cell proliferation assay. Validation of our robotic screening assay for EA should be commercially important because of the large number of chemicals (>10,000) - much less chemical mixtures -that should be screened for EA by profit, non-profit, or governmental entities. Data from our robotic assay will also be of commercial importance because it will enable us to design proprietary formulations of additives that lack EA for foodstuffs and plastics.

最近的科学研究表明，用于塑料，药物，埃斯蒂酰胺，化妆品，食品添加剂等的许多化学物质都是内分泌干扰物（EDS）。 EDS以各种方式干扰荷尔蒙活性，以对许多生理过程产生严重的不利影响（皮摩尔至纳摩尔）浓度，尤其是在胎儿或发育中的哺乳动物上。 ED在我们环境中的流行率和行动值得开发高度具体，敏感，可靠，快速和成本效益的检测。因此，各种政府机构（EPA，FDA和ICCVAM）和积极主动的公司明确希望对EA进行体外机器人测定 可用，验证得多。 认证（CCI）刚刚成功完成了I期SBIR项目，以证明使用MCF-7细胞增殖为EA开发机器人测定是可行的。在此II阶段应用中，CCI提议改进和广泛验证该测定法，以便可以在第三阶段中完全商业化。 CCI还建议检查EA对食品和塑料中使用的一组抗氧化剂的理论预测与该机器人细胞增殖测定法测量的实际EA之间的关系。 验证我们对EA的机器人筛选测定法应该在商业上很重要，因为大量化学药品（> 10,000）（> 10,000） - 化学混合物少得多 - 应通过利润，非营利组织或政府实体来筛选EA。我们机器人测定法的数据也将具有商业意义，因为它将使我们能够设计缺乏EA的专有配方，这些添加剂缺乏食品和塑料。