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An In Vitro Robotic Assay for Estrogenic Activity

雌激素活性的体外机器人测定

基本信息

批准号：
6446575
负责人：
Cathy Z Yang
金额：
$ 9.1万
依托单位：
CERTICHEM, INC.
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-03-01 至 2003-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6446575
关键词：
biosensor device biotechnology cell proliferation endocrine disorder estrogens high throughput technology hormone metabolism hormone regulation /control mechanism robotics technology /technique development

项目摘要

DESCRIPTION (provided by applicant): Recent scientific investigations have shown that many chemicals used in plastics, pharmaceuticals, pesticides, cosmetics, food additives, etc., are endocrine disruptors that interfere in various ways with the action of estrogen, androgen, or thyroid hormones. The most common endocrine effects of disruptor involve estrogenic activity that can have significant deleterious effects on many physiological processes at very low (picomolar to nanomolar) concentrations, especially on fetal or developing mammals. The prevalence and actions of endocrine disruptors in our environment warrants the development of highly specific, sensitive, reliable, rapid, and cost-effective detection. A high throughput robotic cell proliferation assay would constitute such a test. Hence, in this SBIR Phase I proposal, CertiChem proposes to develop a high throughput robotic assay to detect estrogenic activity by measuring in vitro proliferation of MCF-7 cells. Such a robotic screening assay should be commercially important because of the large number of chemicals (> 10,000) and mixtures of chemicals that should be screened for estrogenic activity by profit, non-profit, or governmental entities. Our preliminary data demonstrate that CertiChem has the expertise to develop this in vitro robotic assay for estrogenic activity. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（由申请人提供）：最近的科学研究表明许多用于塑料、药品、杀虫剂化妆品、食品添加剂等，是内分泌干扰物，通过雌激素、雄激素或甲状腺激素的各种作用方式。的干扰物最常见的内分泌效应涉及雌激素活性，对许多生理过程有显著的有害影响，低浓度（皮摩尔至纳摩尔），尤其是在胎儿或发育中哺乳动物环境中内分泌干扰物的流行和作用保证开发高度特异性、敏感性、可靠性、快速性和具有成本效益的检测。高通量机器人细胞增殖测定会构成这样的测试。因此，在SBIR第一阶段提案中，CertiChem 建议开发一种高通量机器人检测雌激素通过测量MCF-7细胞的体外增殖来测定活性。这种机器人筛选测定在商业上应该是重要的，因为大量的化学品（> 10，000）和化学品混合物，应筛选营利、非营利或政府实体的雌激素活动。我们初步数据表明，CertiChem拥有开发这一技术的专业知识，用于雌激素活性体外机器人测定。拟议商业应用：不可用