CELLULAR & COMPUTATIONAL ANALYSIS OF ODOR LEARNING

蜂窝网络

• 批准号: 6910903
• 负责人: Alan Gelperin
• 金额: $ 15.18万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6910903
• 关键词: Gastropoda; behavior test; computer simulation; conditioning; memory; olfactions; olfactory lobe; olfactory stimulus; sensory discrimination

DESCRIPTION (Adapted from the Investigator's Abstract) The long-term objective of this proposal is to understand the cellular and biochemical basis of long-term memory storage, using the storage of odor memories in the olfactory system of a terrestrial mollusk as a model system. We will test a specific cellular and biophysical model for how the major central site of odor information processing in the terrestrial slug Limax maximus forms odor memories as spatially segregated bands of neurons. The odor memory storage site, the procerebral (PC) lobe, is analogous to the mammalian olfactory bulb. The hypothesis to be tested is that olfactory memories are identified by odor learning-dependent uptake of a highly fluorescent dye, Lucifer yellow, by groups of neurons in the PC lobe. Learning-dependent dye uptake identifies the neurons involved in storing the odor memory for detailed biophysical and biochemical analysis. The specific aims of the proposed work will test several predictions of the hypothesis that the learning-dependent dye labeling identifies neurons storing an odor memory. The following specific experimental questions will be addressed; 1. If the slug is denied access to the dye-labeled neurons in the PC lobe after odor training, does it act naive in a behavioral test of odor memory function? 2. Does the spacing between two dye-labeled bands produced by learning about two odors predict the ability to discriminate between the two learned odors? 3. Are the dye labeled neurons present after odor training selectively activated by stimulation with the trained odor? 4.Do drugs which disrupt normal dynamics of neuronal activity in the PC lobe also degrade odor learning and odor discrimination? The computational model which leads to the specific experimental tests listed in the specific aims will be expanded to incorporate more specific details of the cellular biophysics and neuron connectivity in the PC lobe. We also will study the cellular mechanism of learning-dependent dye uptake to develop this memory localization tool for use in mammalian brain. The conserved nature of biochemical memory mechanisms and system design principles in olfactory systems makes it very likely that mechanisms for odor memory storage in the molluscan nervous system will have direct relevance to the mammalian nervous system. The results of this work will contribute to efforts to develop pharmacological interventions for clinical syndromes in which loss of memory function is a major component, such as mental retardation and Alzheimers disease.

描述（改编自研究者摘要） 这项提案的长期目标是了解细胞和 长期记忆储存的生化基础，利用气味的储存 作为模型系统的陆生软体动物的嗅觉系统中的记忆。我们 将测试一个特定的细胞和生物物理模型，以了解主要的中枢神经系统是如何 陆栖蛞蝓Limax maximus形态中气味信息处理的位点 气味记忆是空间分离的神经元带。气味记忆存储 前脑叶（PC）类似于哺乳动物的嗅球。 有待检验的假设是，嗅觉记忆是通过气味来识别的 高荧光染料，荧光黄的学习依赖性摄取， PC叶中的神经元群。学习依赖的染料摄取确定了 神经元参与储存气味记忆的详细生物物理和 生化分析拟议工作的具体目标将测试几个 预测的假设，学习依赖的染料标记 识别储存气味记忆的神经元。以下具体实验 问题将得到解决; 1。如果子弹被拒绝进入染料标记的 在气味训练后PC叶的神经元，它是否在行为上表现幼稚， 气味记忆功能测试2.两个染料标记带之间的间隔 通过学习两种气味产生的气味预测辨别能力， 两种气味之间的区别吗3.是否有染料标记的神经元存在于 气味训练选择性激活刺激训练气味？4.Do 破坏PC叶中神经元活动的正常动力学的药物也 降低气味学习和气味辨别能力计算模型， 导致在具体目标中列出的具体实验测试将是 扩展到包含细胞生物物理学的更具体的细节， PC叶的神经元连接。我们还将研究细胞机制 学习依赖的染料摄取来开发这种记忆定位工具， 用于哺乳动物大脑。生物化学记忆机制的保守性 嗅觉系统中的系统设计原理使得它很可能 软体动物神经系统中的气味记忆储存机制 与哺乳动物的神经系统直接相关。这项工作的结果将 有助于努力开发药理学干预， 记忆功能丧失是主要组成部分的综合征，如精神 发育迟缓和阿尔茨海默病。