A gene map for Biomphalaria glabrata, a S. mansoni host

曼氏沙门氏菌宿主光滑双脐螺 (Biomphalaria glabrata) 的基因图谱

• 批准号: 6955537
• 负责人: MATTY KNIGHT
• 金额: $ 47.9万
• 依托单位: BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6955537
• 关键词: Biomphalaria; Schistosoma mansoni; artificial chromosomes; biotechnology; fluorescent in situ hybridization; genetic mapping; genetic polymorphism; host organism interaction; microarray technology; nucleic acid probes; nucleic acid repetitive sequence; nucleic acid sequence; parasite infection mechanism; polymerase chain reaction; restriction fragment length polymorphism; sequence tagged sites; subtraction hybridization

DESCRIPTION (provided by applicant): The fundamental issue of how a schistosome survives, or is targeted for destruction, in a snail host has important implications for the epidemiology of schistosomiasis. Preceding groundwork clearly showed that genetic variations in the snail Biomphalaria glabrata play a major role in the intramolluscan development of the parasitic helminth Schistosoma mansoni. Snail stocks that were genetically selected as either parasite resistant or susceptible have provided us with valuable resources for identifying markers as well as gene(s) important in the snail/parasite relationship. Our objective is to use well-established molecular tools, studying defined snail stocks with stable but different susceptibility phenotypes, to identify and characterize those snail genes that play a critical role in resisting, or fully supporting, a schistosome infection. Polymorphisms in resistant vs. susceptible snail DNA should enable us to efficiently isolate probes targeting those sequences involved in the snail/parasite relationship. Use will also be made of examining the heritability of polymorphic markers in progeny snails that segregate for resistance or susceptibility. By generating suppression subtraction hybridization (SSH) libraries from exposed vs normal resistant and susceptible juvenile snails and, by screening an available snail microarray with cDNA probes from resistant and susceptible juvenile snails ( parasite infection), we hope to identify genes that govern these different infection phenotypes in this snail-host. For positional mapping of B. glabrata chromosomes by FISH, we will use (as probes) BAG clones, corresponding to transcripts identified from the microarray/subtraction studies. Our overall strategy is to reduce the inflow of repetitive markers and combine useful information gained at both DNA and RNA levels to dissect relevant gene products involved in parasite development or destruction coded for, within the relatively large B. glabrata genome.

描述（由申请人提供）：血吸虫如何在蜗牛宿主中存活或被靶向破坏的基本问题对血吸虫病的流行病学具有重要意义。前面的基础清楚地表明，在蜗牛的遗传变异Biomphalaria光滑发挥了重要作用，在软体动物内的寄生蠕虫曼氏血吸虫的发展。通过遗传学选择的抗寄生虫或易感寄生虫的蜗牛种群为我们提供了宝贵的资源，用于鉴定在蜗牛/寄生虫关系中重要的标记和基因。我们的目标是使用成熟的分子工具，研究具有稳定但不同易感性表型的限定蜗牛种群，以鉴定和表征那些在抵抗或完全支持寄生虫感染中起关键作用的蜗牛基因。抗性与易感蜗牛DNA的多态性应使我们能够有效地分离探针，靶向那些序列参与蜗牛/寄生虫的关系。还将利用遗传性的多态性标记的后代蜗牛分离的电阻或敏感性进行检查。通过产生抑制消减杂交（SSH）库暴露与正常的抗性和敏感的少年蜗牛，并通过筛选可用的蜗牛微阵列与cDNA探针从抗性和敏感的少年蜗牛（寄生虫感染），我们希望确定基因，这些不同的感染表型在这个蜗牛主机。用于B的位置映射。为了通过荧光原位杂交技术对glabrata染色体进行测序，我们将使用（作为探针）BAG克隆，其对应于从微阵列/扣除研究中鉴定的转录本。我们的总体策略是减少重复标记的流入，并将在DNA和RNA水平上获得的有用信息联合收割机在相对较大的B内解剖参与寄生虫发育或破坏编码的相关基因产物。glabrata基因组