Development of Novel Robust Laundry Enzymes for Cold Wash Applications by both rational and directed evolution and using micro-fluidic approaches

该项目将涉及从极端细菌和古细菌中识别具有改进性能的新型酶，用于洗涤剂和医疗保健部门。特别是既耐用又具有高活性的酶，可用于可持续的冷水洗涤。之前的研究使用了从嗜冷菌中分离出来的酶。这种方法有其局限性，因为尽管酶在低温下是活跃的，但它们不足以用于洗衣目的。该方法将包括使用坚固的酶支架和灵活的活性部位，使其在常温下具有高活性。这一方法将通过理性和定向进化以及使用微流体方法来实现。

The project will be involved in identifying novel enzymes from extremophilic bacteria and archaea that have improved properties for use in the detergent and healthcare sector. Specifically enzymes that are both robust and have high activity for sustainable cold water washing. Previous studies have used enzymes isolated from psychrophilic bacteria. This approach has its limitations since although the enzymes are active at low temperatures they are not robust enough for laundry purposes. The approach will involve using a robust enzyme scaffold together with a flexible active site that will allow high activity at ambient temperatures. This approach will be carried out by both rational and directed evolution and using micro-fluidic approaches.