Neuropathology of dopamine systems in schizophrenia

描述(由申请人提供)：本申请是一项补充资金申请，用于购买一些设备和软件，以改善我们已经由NIH资助的基金：精神分裂症中多巴胺系统的神经病理学(基金编号：MH66123)的开发和结果。在我们的研究中使用了相当多的不同设备，其中一些几乎只由罗伯茨博士实验室的人员使用(即电子显微镜、电子显微镜的自动打印机等)。其他设备在MPRC共享(即我们的赠款中使用的体视学软件和显微镜)。自从最初的赠款提交以来，用户的数量和这一共享显微镜和立体系统的使用时间已经大幅减少。目前，我们每周只能使用一次这种设备(这包括使用显微镜和成像分析软件的时间)，这造成了我们项目所有光学显微镜分析的非常显著的延迟。此外，在过去的几个月里，我们的数据进展表明，引入一些新的方法可以改善我们的项目。事实上, 我们已经做了一项初步研究，以确定免疫荧光技术是否对我们的 学习。如下面几页所示，在我们的项目中使用免疫荧光技术有几个优点，还将使我们能够分析一些用更传统的技术无法分析的数据。由于所有这些原因，在本申请中，我们要求提供资金，以装备用于荧光和体视学研究的新显微镜，以及对所获得的数据进行分析所需的软件。这将对我们在原始拨款中提议的至少4个实验产生积极影响。我们认为，这些收购将大大改善数据分析和我们研究的结果，包括更快地分析和更准确地解释所获得的结果。

DESCRIPTION (provided by applicant): The present application is a request for supplemental funding for the acquisition of some equipment and software that can improve the development and outcome of our already NIH funded grant entitled: Neuropathology of dopamine systems in schizophrenia (grant number: MH66123). In our study a considerable number of different equipment is used, some of which is almost exclusively used by personnel in Dr. Roberts laboratory (i.e. electron microscope, automated printer for electron microscopy, etc.) Other equipment is shared at MPRC (i.e. the software and microscope for stereology used in our grant). Since the submission of the original grant, the number of users and the time that this shared microscope and stereology system is available to use have decreased dramatically. Currently we are allowed to use this equipment only once a week (this includes the time for use of the microscope and also the imaging analysis software), creating a very striking delay in all the light microscopy analysis of our project. Furthermore, in the last few months our data progress indicates that the introduction of some new methodology can improve our project. In fact, we have done a preliminary study to determine if immunofluorescence techniques will be useful for our study. As it is shown in the next pages the use of immunofluorescence techniques in our project presents several advantages and also will allow us to analyze some data that would not be possible to analyze with more traditional techniques. For all these reasons in the present application we request funding for a new microscope equipped for fluorescence and stereology studies, as well as the software necessary to perform the analysis of the date obtained. This will positively impact at least 4 of our experiments proposed in the original grant. We consider that these acquisitions will considerably improve the data analysis and the outcome of our study, including a faster analysis and more accurate interpretation of the results obtained.