Neuropathology of dopamine systems in schizophrenia

描述（由申请人提供）：本申请是一项补充资金购买一些设备和软件的请求，这些设备和软件可以改善我们已经由 NIH 资助的拨款项目的开发和结果，题为：精神分裂症中多巴胺系统的神经病理学（拨款号：MH66123）。在我们的研究中使用了相当多的不同设备，其中一些设备几乎完全由罗伯茨博士实验室的人员使用（即电子显微镜、电子显微镜自动打印机等）。其他设备在 MPRC 共享（即我们资助中使用的体视学软件和显微镜）。自提交原始拨款以来，该共享显微镜和体视学系统的用户数量和可用时间已大幅减少。目前，我们每周只能使用该设备一次（这包括使用显微镜和成像分析软件的时间），这对我们项目的所有光学显微镜分析造成了非常显着的延迟。此外，在过去几个月中，我们的数据进展表明，引入一些新方法可以改进我们的项目。事实上， 我们已经做了一项初步研究，以确定免疫荧光技术是否对我们有用 研究。正如下一页所示，在我们的项目中使用免疫荧光技术具有多种优势，并且还使我们能够分析一些使用更传统的技术无法分析的数据。出于所有这些原因，在本申请中，我们请求资助用于荧光和体视学研究的新型显微镜，以及对所获得的数据进行分析所需的软件。这将对我们在原始资助中提出的至少 4 项实验产生积极影响。我们认为这些收购将大大改善数据分析和我们的研究结果，包括更快地分析和更准确地解释所获得的结果。

DESCRIPTION (provided by applicant): The present application is a request for supplemental funding for the acquisition of some equipment and software that can improve the development and outcome of our already NIH funded grant entitled: Neuropathology of dopamine systems in schizophrenia (grant number: MH66123). In our study a considerable number of different equipment is used, some of which is almost exclusively used by personnel in Dr. Roberts laboratory (i.e. electron microscope, automated printer for electron microscopy, etc.) Other equipment is shared at MPRC (i.e. the software and microscope for stereology used in our grant). Since the submission of the original grant, the number of users and the time that this shared microscope and stereology system is available to use have decreased dramatically. Currently we are allowed to use this equipment only once a week (this includes the time for use of the microscope and also the imaging analysis software), creating a very striking delay in all the light microscopy analysis of our project. Furthermore, in the last few months our data progress indicates that the introduction of some new methodology can improve our project. In fact, we have done a preliminary study to determine if immunofluorescence techniques will be useful for our study. As it is shown in the next pages the use of immunofluorescence techniques in our project presents several advantages and also will allow us to analyze some data that would not be possible to analyze with more traditional techniques. For all these reasons in the present application we request funding for a new microscope equipped for fluorescence and stereology studies, as well as the software necessary to perform the analysis of the date obtained. This will positively impact at least 4 of our experiments proposed in the original grant. We consider that these acquisitions will considerably improve the data analysis and the outcome of our study, including a faster analysis and more accurate interpretation of the results obtained.