Micro-and Nano-engineering of Biomineralized Materials

生物矿化材料的微纳米工程

• 批准号: 6932040
• 负责人: W. Mark Saltzman
• 金额: $ 27.71万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932040
• 关键词: X ray crystallography; bioengineering /biomedical engineering; biomaterial development /preparation; biomaterial evaluation; biomaterial interface interaction; biotechnology; bone; bone regeneration; cell adhesion; cell membrane; cell migration; cell morphology; cell proliferation; clinical research; confocal scanning microscopy; dental materials; electron microscopy; human tissue; macromolecule; minerals; molecular film; nanotechnology; tissue engineering; tissue support frame

DESCRIPTION (provided by applicant): Biomaterials have a long and successful history in dental and bone restoration, but the current technology is imperfect. Three-dimensional, biomineralized templates are a necessary component of bone and dental tissue engineering strategies, but methods do not yet exist for controlling the formation of biomineralized substrates with chemical and physical features that promote cell integration and function. We will first fabricate two-dimensional substrates in which macromolecules with functional groups that appear during tooth and bone formation will be micropatterned to regulate calcium phosphate crystal nucleation and growth. A constant composition method will be used to mineralize the patterned surfaces with specific chemical composition and crystalline phase. In addition, three-dimensional matrices with micropatterns will be fabricated using natural ECM proteins or synthetic polymers in a silicon mold. The patterned hydrogel matrices will be mineralized in a controlled manner using a modified constant composition method and microfluidics. Mineralized structures will be extensively characterized for chemistry, crystal structure, and topography. The controlled microstructures will be used to study the interaction between cells and the extracellular environment. Experiments will focus on the effect of the mineralized material on cell behavior; the influence of chemistry and geometry on the attachment, migration, and specific functions of cells will be studied. Together, these studies will provide new insight on the interactions between cells and substrates that occur during biomineralization. Since precisely designed synthetic materials will be produced and characterized to meet the study objectives, this project will also lead to new approaches for engineering of dental and orthopedic tissue.

描述（申请人提供）：生物材料在牙齿和骨骼修复方面有着悠久而成功的历史，但目前的技术还不完善。三维生物矿化模板是骨和牙齿组织工程策略的必要组成部分，但目前还没有方法来控制具有促进细胞整合和功能的化学和物理特征的生物矿化基质的形成。