ACTIN ASSEMBLY DURING YEAST POLARIZED CELL GROWTH

超出所提供的空间。细胞极化对于许多生理过程如胚胎发育、细胞运动和细胞分化是至关重要的。阐明细胞极性建立的机制对于理解发育异常和疾病如侵袭性肿瘤和动脉硬化具有重要意义。鉴定细胞极性产生的特异性分子可能揭示这些疾病的新治疗靶点。此外，细胞如何打破对称性并建立极性是细胞生物学中的一个深刻问题。了解细胞的内在能力，以调节细胞的组织和形态发生的物理原理的基本见解，在许多真核细胞类型的细胞极性的一个关键调节因子是Rho家族的GTdcdc 42。在细胞极化过程中，Cdc 42以局部方式激活并诱导下游事件，最重要的是，产生局部肌动蛋白细胞骨架元件。这个提议的目的是了解Cdc 42如何诱导肌动蛋白丝的极化聚合。我们将继续我们正在进行的工作，研究肌动蛋白丝是如何在细胞皮层成核和Cdc 42如何定义在细胞极化过程中的肌动蛋白成核的网站。我们还将研究Cdc 42的激活形式驱动细胞对称性初始破坏的机制。具体目标是：1）Beel/Vrpl/肌球蛋白-I复合物的生物化学表征; 2）研究细胞极化过程中Beel/Vrpl/肌球蛋白-I复合物的调节; 3）理解Arp 2/3复合物使肌动蛋白聚合成核的机制; 4）研究驱动细胞极性产生的内在机制。性能现场=

EXCEED THE SPACE PROVIDED. Cell polarization is fundamentally important for many physiological processes such as embryonic development, cell motility and cell differentiation. Elucidation of the mechanism underlying cell polarity establishment is important for understanding developmental abnormalities and diseases such as invasive tumor and arteriosclerosis. Identification of the molecules that are specialized in cell polarity generation may reveal novo therapeutic targets for these diseases. In addition, how cells break symmetry and establish a polarity is a profound problem in cell biology. Understanding cell's intrinsic ability to polarize may lead to fundamental insights into the physical principles that govern cellular organization and morphogenesis A key regulator of cell polarity in many eukaryotic cell types is the Rho-family GTPase Cdc42. During cell polarization, Cdc42 is activated in a localized manner and induces downstream events, most importantly, the generation of localized actin cytoskeletal elements. The goal of this proposal is to understand how Cdc42 induces polarized polymerization of actin filaments. We will continue our ongoing work studying how actin filaments are nucleated at cell cortex and how Cdc42 defines the site of actin nucleation during cell polarization. We will also investigate the mechanism by which the activated form of Cdc42 drives the initial breakage of cell symmetry. The specific aims are: 1) Biochemical characterization of the Beel/Vrpl/myosin-I complex; 2) Studying the regulation of the Beel/Vrpl/myosin-I complex during cell polarization; 3) Understanding the mechanism by which the Arp2/3 complex nucleate actin polymerization; 4) Investigating the Intrinsic mechanism that drives cell polarity generation. PERFORMANCE SITE ========================================Section End===========================================