Recording/Stimulation of Stepping in Spinal Cord L1-l2

记录/刺激脊髓 L1-l2 的步进

• 批准号: 6855701
• 负责人: JACK W JUDY
• 金额: $ 14.06万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6855701
• 关键词: ankle; biomechanics; brain electrical activity; brain stem; chordate locomotion; decerebration; electrodes; electromyography; electrostimulus; implant; laboratory rat; mesencephalon; neural information processing; neuroanatomy; spinal cord; statistics /biometry; video recording system

DESCRIPTION (provided by applicant): Spinal cord injury affects upwards of 230,000 people and can cost between $487,150 and $2,185,667 per person. Interneuronal networks within the L1-L2 region of the spinal cord are intrinsically capable of coordinating the activation of multiple functional muscle groups as is required in locomotion. By tapping into this network using intraspinal microstimulation within the spinal cord, simple, natural, and adaptable control of locomotion may be achievable. In order to identify the key regions and timing of activation, we will simultaneously record the extracellular activity at an array of sites within the L1-L2 region during stepping to create a spatiotemporal population map. Recent advances in neuronal ensemble recording make it possible to look at the spatial and temporal properties of neuronal networks in vivo. We will record from the L1-L2 region of the spinal cord using high-density-multielectrode arrays during locomotion, and will correlate the spatiotemporal profile with electromyographic (EMG) data, videography, and ankle position/force measurements. Locomotion will be studied in acute decerebrate rats by stimulation of the mesencephalic Iocmotor region and in rats that have been chronically implanted with a multielectrode array for recording and stimulation on a treadmill. Using principal component analysis and independent component analysis, we will characterize the overall neural activity and identify neurons that are highly correlated with specific phases of the step cycle or rate of stepping. Then we will determine the dynamic interdependence of these sets using directed coherence to determine which sites are appropriate targets. The efficacy of these sequences will be explored by electrically stimulating the implanted multielectrodes in spinal transected rats. To do this, a 96- channel multielectrode stimulation system capable of outputting a spatially and temporally patterned stimulus is being developed. The results of these studies could lead towards the development of models for activation of the lumbar circuits in humans and could be used in combination with treadmill training, pharmacological intervention, functional nerve stimulation strategies, and other neural repair therapies, for the restoration of locomotion.

描述（由申请人提供）：脊髓损伤影响超过23万人，每人的费用在487,150美元至2,185,667美元之间。脊髓L1-L2区域内的神经元间网络具有协调多个功能肌群激活的内在能力，这是运动所需要的。通过利用脊髓内的椎管内微刺激进入这个网络，可以实现简单、自然和适应性强的运动控制。为了确定激活的关键区域和时间，我们将在步进过程中同时记录L1-L2区域内一系列位点的细胞外活动，以创建时空种群图。神经元集合记录的最新进展使观察活体神经元网络的时空特性成为可能。我们将在运动过程中使用高密度多电极阵列记录脊髓L1-L2区域，并将时空剖面与肌电图（EMG）数据、录像和踝关节位置/力测量相关联。通过刺激中脑运动区和长期植入多电极阵列在跑步机上记录和刺激的急性失脑大鼠的运动将被研究。使用主成分分析和独立成分分析，我们将描述整体神经活动，并识别与步进周期或步进速率的特定阶段高度相关的神经元。然后，我们将使用定向一致性来确定这些集合的动态相互依存关系，以确定哪些站点是合适的目标。这些序列的有效性将通过电刺激在脊髓横断的大鼠中植入的多电极来探索。为此，一种能够输出空间和时间模式刺激的96通道多电极刺激系统正在开发中。这些研究的结果可能导致人类腰椎回路激活模型的发展，并可与跑步机训练、药物干预、功能性神经刺激策略和其他神经修复疗法结合使用，以恢复运动能力。