Defining the prion domain of PrP

定义 PrP 的朊病毒结构域

• 批准号: 6870268
• 负责人: JAMES A MASTRIANNI
• 金额: $ 34.9万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-15 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6870268
• 关键词: SDS polyacrylamide gel electrophoresis; clinical research; confocal scanning microscopy; fluorescence resonance energy transfer; genetically modified animals; human tissue; laboratory mouse; neural degeneration; postmortem; posttranslational modifications; prions; protein sequence; protein structure function; spongiform encephalopathy; tissue /cell culture

DESCRIPTION (provided by applicant): The prion diseases are a family of transmissible neurodegenerative disorders that affect humans and animals. A large body of evidence argues that a post-translational, non-covalent modification of the prion protein (PrP) is the fundamental event in the mechanism underlying these diseases. The normal cellular isoform (PrPC) is misfolded to the beta-sheet rich pathogenic isoform (PrPSc). Once formed, PrPSc appears to act as a conformational template to convert additional PrPC to PrPSc. Considerable evidence supports sequence homology within the central region of PrP as a necessary feature in the association of PrPSc with PrPC as a prelude to conversion and propagation of PrPSc, but the exact segment(s) involved and the sequence determinants for this conversion are unknown. Studies targeted at understanding the site(s) of association of PrPC and PrPSc will no doubt define ways to inhibit their interaction and provide treatment for these currently untreatable diseases. The proposed studies are designed, therefore, to define the molecular determinants within PrP, and primarily within the putative priori domain, that are required for efficient self-association and conformational transfer. We will primarily utilize transgenic mice that express polymorphic PrP genes to act as hosts for a variety of sporadic and genetic human prion diseases to determine if and where homologous regions and residues are required for efficient transmission of prion strain. In addition a novel yeast-based model of prion disease will be extensively utilized to study the effect of specific substitutions or deletions at potentially critical association sites of PrP on the development of PrPSC-like protein. This powerful model is not only capable of generating prpSC-Iike protein, but can support the de novo generation of at least two strains of PrPSc so, and demonstrate conformational transference of PrPSc to PrPC. The information gained from these studies will then be applied to the development of novel peptide inhibitors that are designed to bind to critical sites within the defined "prion domain" and halt additional binding of PrP. With the continued threat of bovine spongiform encephalopathy in Europe, and the current spread of chronic wasting disease of deer and elk in the U.S., these studies are urgently needed.

描述(申请人提供)：Pron病是一种影响人类和动物的遗传性神经退行性疾病家族。大量证据表明，蛋白质的翻译后非共价修饰(PrP)是导致这些疾病的机制中的基本事件。正常细胞亚型(PrPC)被错误折叠为富含β-折叠的致病亚型(PrPSc)。一旦形成，PrPSc看起来就像一个构象模板，将额外的PrPC转化为PrPSc。相当多的证据支持PrP中心区的序列同源性是PrPSc与PrPC结合的必要特征，这是PrPSc转换和繁殖的前奏，但所涉及的确切片段(S)和这种转换的序列决定因素尚不清楚。旨在了解PrPC和PrPSc结合部位(S)的研究无疑将确定抑制它们相互作用的方法，并为这些目前无法治疗的疾病提供治疗。因此，拟议的研究旨在定义PrP中的分子决定因素，主要是在假定的先验结构域中，这些决定因素是有效的自结合和构象转移所必需的。我们将主要利用表达多态PrP基因的转基因小鼠作为各种散发性和遗传性人类普恩疾病的宿主，以确定是否以及在哪里需要同源区域和残基来有效传播普恩病毒株。此外，一种新的基于酵母的PrP疾病模型将被广泛用于研究PrP潜在关键结合位点的特定替换或缺失对PrPSC样蛋白发展的影响。这个强大的模型不仅能够产生PrpSC样蛋白，而且可以支持至少两株PrPSc SO的从头产生，并证明PrPSc向PrPC的构象转移。然后，从这些研究中获得的信息将被应用于新型多肽抑制剂的开发，这些多肽抑制剂被设计成与已定义的“Pron结构域”内的关键位点结合，并阻止PrP的额外结合。随着牛海绵状脑病在欧洲的持续威胁，以及目前美国鹿和麋鹿慢性消耗性疾病的传播，这些研究是迫切需要的。