RAT RESOURCE AND RESEARCH CENTER: CLONING TECHNOLOGY

大鼠资源与研究中心：克隆技术

• 批准号: 7153957
• 负责人: Philip M Iannaccone
• 金额: $ 19.72万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7153957
• 关键词: animal breeding; animal colony; biotechnology; disease /disorder model; laboratory rat

DESCRIPTION (Adapted from the applicant's abstract): Rats as models for human disease have made valuable contributions to almost every field of human medicine. Recently, major advances have been made in developing reagents, genomic information, and critical germline modification techniques for the rat, including making transgenic animals. With these tools in place, rat models can make unprecedented contributions toward understanding the molecular basis of human disease and targeting development of new therapeutic strategies. Unfortunately, there are several major impediments to realization of the full potential of rats as models of human disease: 1) Many inbred and genetically engineered rat strains are not available to researchers. While commercial producers maintain inventories of commonly used rat strains, less commonly used strains are frequently unavailable to researchers. 2) While individual investigators develop and characterize many mutant rat models, they lack the physical and financial resources to maintain potentially important strains. 3) Gene knockouts are not currently possible in the rat. 4) Quality control with respect to infectious diseases is often lacking. Taken together, these impediments document the clear need for a resource center that can provide high quality rat strains to the biomedical community and develop techniques needed for advancement of rats as models. The overall goal of this application is to establish a Rat Resource & Research Center (RRRC). This center will meet the needs of the biomedical research community by developing the infrastructure for distributing high quality, well-characterized inbred, hybrid and genetically engineered rat (GER) to investigators, either as live rats or as cryopreserved germplasm, and by performing innovative research that generates new information which will benefit the RRRC and the scientific community. To accomplish this goal, an established multidisciplinary consortium: the Cryobiology Research Institute at Indiana University (CRI), Harlan Sprague Dawley Inc. (HSD), Memorial Institute for Education and Research (CMIER) at Northwestern University Medical School, will develop a RRRC. This unique consortium has extensive expertise in each of the disciplines needed, including importation, rederivation, breeding, embryo and gamete cryopreservation, genotyping, phenotyping, infectious disease monitoring, and distribution of laboratory rats. The RRRC will provide an uninterrupted supply of rats to biomedical investigators. Research projects aimed at improving rat nuclear transfer and developing rat knockout models, cryobiology, and health monitoring of rats will be performed. Results will benefit investigators worldwide.

描述（改编自申请人摘要）：大鼠作为人类模型 疾病对人类几乎所有领域都作出了宝贵贡献 药 最近，在开发试剂方面取得了重大进展， 基因组信息和关键的生殖系修饰技术， 包括制作转基因动物。 有了这些工具，老鼠 模型可以为理解分子生物学做出前所未有的贡献。 人类疾病的基础和新疗法的靶向开发 战略布局 不幸的是，实现这一目标有几个主要障碍 大鼠作为人类疾病模型的全部潜力：1）许多近交系和 研究人员无法获得基因工程大鼠品系。 而 商业生产者保持常用鼠种的库存， 研究人员经常得不到常用的菌株。 2)而 个别研究人员开发和表征许多突变大鼠模型，他们 缺乏物质和财政资源来维持潜在的重要性， 菌株 3)基因敲除目前在大鼠中是不可能的。 4)质量 对传染病往往缺乏控制。 综合起来看， 这些障碍表明，显然需要一个资源中心， 为生物医学界提供高质量的大鼠品系， 需要的技术来提高大鼠作为模型的地位。 总的目标是 申请的目的是建立一个大鼠资源和研究中心（RRRC）。 这 中心将满足生物医学研究界的需求， 基础设施，用于分配高质量，良好的特点近交， 杂交和基因工程大鼠（格尔），无论是作为活体 大鼠或作为冷冻保存的种质，并通过进行创新研究， 产生新的信息，这将有利于RRRC和科学 社区 为了实现这一目标，建立了多学科 财团：印第安纳州大学低温生物学研究所（CRI）， 哈兰斯普拉格道利公司(HSD)，纪念教育学院和 西北大学医学院的研究（CMIER）将开发一种 RRRC。 这个独特的财团在每个领域都有广泛的专业知识， 需要的学科，包括进口，再衍生，育种，胚胎和 配子冷冻保存，基因分型，表型，传染病 实验室大鼠的监测和分布。 RRRC将提供 不间断地向生物医学研究者提供老鼠。 研究项目 旨在改进大鼠核移植和开发大鼠基因敲除模型， 将进行大鼠的低温生物学和健康监测。 结果将 全世界的研究人员。