THE XIPHOPHORUS GENETIC STOCK CENTER

剑尾鱼遗传库存中心

• 批准号: 7153958
• 负责人: RONALD B WALTER
• 金额: $ 35.35万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7153958
• 关键词: Internet; animal colony; artificial chromosomes; computer program /software; computer simulation; computer system design /evaluation; cryopreservation; fish; freezing; genetic library; genetic mapping; genetic markers; genetic polymorphism; genetic registry /resource /referral center; genetic strain; genome; genotype; information dissemination; information systems; method development; molecular cloning; polymerase chain reaction; quantitative trait loci; sperm

This is a P40 resource proposal to maintain and expand the Xiphophorus Genetic Stock Center (XGSC), and enhance services provided to research investigators. Xiphophorus is a small freshwater, aquarium fish that has been extensively used in carcinogenesis studies, but also used as a model organism in a variety of different fields of study that transcends several NIH institutes. The XGSC has operated within the United States for over 70 years. The proposed initiatives will greatly enhance the usability of the Xiphophorus model system for research scientists using this system, but also for "non-Xiphophorus" scientists that envision application of the powerful genetics provided by this model system. The traditional strength of this model involves the non-biased assessment of genetic variability among Xiphophorus species (22 known taxa), and capability of producing fertile interspecies hybrids, allows chromosomal/marker inheritance to be followed in individual backcross hybrid progeny. Complex traits, such as susceptibility to the development of neoplastic disease, or behavioral characteristics, can be studied using Xiphophorus, and underlying genetic factors can be identified. Since research using these fishes is currently expanding within the scientific community, it is timely to enhance the services offered by the Xiphophorus Genetic Stock Center. We propose: 1) To preserve and enhance the Xiphophorus Genetic Stock Center and provide the research community with animals from genetically managed lines in large numbers according to the specifications of each investigator. 2) To establish and maintain extensive and flexible computer databases of fish, and derived samples. These databases will be made available for scientific use. Links and information regarding fish maintenance, current research projects and other XGSC information will be provided via the xiphophorus.org web- site. 3) To continue saturation of the Xiphophorus gene map and development of technologies providing investigators with efficient molecular tools necessary to use this powerful genetic system to approach complex scientific questions, and 4) To establish technology allowing cryopreservation of Xiphophorus sperm samples and thereby provide a preservation program where the XGSC maintains the genetic heritage of all Xiphophorus strains and species.

这是一项P40资源提案，旨在维护和扩大剑尾鱼遗传资源中心（XGSC），并加强为研究人员提供的服务。剑尾鱼是一种小型淡水水族鱼，已被广泛用于致癌研究，但也被用作超越几个NIH研究所的各种不同研究领域的模式生物。 XGSC在美国已经运营了70多年。 拟议的举措将大大提高使用该系统的研究科学家的Xiphophorus模型系统的可用性，也为“非Xiphophorus”的科学家，设想应用该模型系统提供的强大的遗传学。 该模型的传统优势包括剑尾鱼属物种（22种已知类群）之间的遗传变异性的无偏见评估，以及产生可育种间杂交种的能力，允许在个体回交杂交后代中遵循染色体/标记遗传。 复杂的特征，如肿瘤疾病的易感性，或行为特征，可以使用剑尾鱼进行研究，并可以确定潜在的遗传因素。 由于科学界目前正在扩大使用这些鱼类的研究，因此加强剑尾鱼遗传资源中心提供的服务是及时的。我们建议：1）保护和加强剑尾鱼遗传资源中心，并根据每个研究者的具体要求，为研究界提供大量来自遗传管理系的动物。2)建立和维护广泛和灵活的鱼类和衍生样品计算机数据库。这些数据库将供科学使用。 有关鱼类养护、当前研究项目和其他XGSC信息的链接和信息将通过xiphophorus.org网站提供。3)继续饱和的剑尾鱼基因图谱和技术的发展提供必要的研究人员使用这个强大的遗传系统，以接近复杂的科学问题，和4）建立技术允许冷冻保存剑尾鱼精子样本，从而提供一个保存程序，XGSC保持所有剑尾鱼品系和物种的遗传遗产。