Enhanced Development of the Xiphophorus Model System

项目摘要 这一R24建议概述了资源（目标1和2）和研究（目标3）组成部分，旨在 加强剑尾鱼遗传储备中心（XGSC），该中心自2008年以来一直在美国运营。 20世纪30年代，使其成为世界上最古老的活动物资源中心之一，也是国家科学中心。 珍宝般珍贵XGSC目前拥有58个血统遗传系，代表24种剑尾鱼， 在1,400多个水族馆里有活的鱼。XGSC提供了纯种鱼和材料， 来自11个国家的30多个实验室的研究人员。剑尾鱼的传统力量 实验模型涉及与以下相关的遗传遗传模式的无偏见评估： 复杂的表型剑尾鱼种间的高度遗传变异和生产能力 可育的种间杂种允许任何可观察到的性状遗传到个体中。 回交杂种后代例如，肿瘤易感性的遗传控制已经在文献中被记载。 无论是纯品系，并在选择剑尾鱼种间杂交，为各种自发和 诱发的肿瘤，包括几种遗传上不同类型的黑色素瘤。 对剑尾鱼基因组能力的拟议改进有望支持新的基础 关于以下方面的知识：（a）完整生物体内细胞的整体转录状态的变化，（B） 物种间杂交所带来的复杂的基因相互作用，（c）遗传学基础 黑色素瘤易感性，和（d）肿瘤发生的起始和进展。根据我们初步的 数据，我们对下一个项目期间的新科学发现寄予厚望。具体而言是 XGSC将继续为研究界提供动物，增强资源和用户 与遗传管理系相关的数据库，以及来自 所有XGSC线路。基因组组装的增强将通过应用新的光学 作图（BioNano）和超长测序（PacBio）技术。最后，我们将建立基础 主要器官的基因表达谱，并确定基因转导的速度，通过 生物体受到外界刺激后。

Project Summary This R24 proposal outlines both resource (Aims 1 and 2) and research (Aim 3) components designed to enhance the Xiphophorus Genetic Stock Center (XGSC) that has operated within the United States since the 1930's; making it one of the oldest live animal resource centers worldwide and a national scientific treasure. The XGSC currently houses 58 pedigreed genetic lines representing 24 Xiphophorus species of live-bearing fishes in over 1,400 aquaria. The XGSC has provided pedigreed fish and materials to researchers in over 30 laboratories representing 11 countries. The traditional strength of the Xiphophorus experimental model involves the non-biased assessment of genetic inheritance patterns associated with complex phenotypes. The high genetic variability among Xiphophorus species and capability of producing fertile interspecies hybrids allows inheritance of any observable trait to be followed into individual backcross hybrid progeny. For example, genetic control of tumor susceptibility has been documented in both pure strains and in select Xiphophorus interspecies hybrids, for a variety of spontaneous and induced neoplasms, including several genetically distinct types of melanoma. Proposed improvements to Xiphophorus genomic capabilities promises to support new fundamental knowledge regarding; (a) shifts in the global transcriptional state of cells within the intact organism, (b) complex gene interactions brought about by interspecies hybridization, (c) the genetics underlying melanoma susceptibility, and (d) the initiation and progression of tumorigenesis. Given our preliminary data, we have high expectations for novel scientific findings in the next project period. Specifically, the XGSC will continuing to provide the research community with animals, enhanced resources, and user databases related to the genetically managed lines, and complete cryopreservation of germplasm from all XGSC lines. Enhancements to the genome assemblies will be made by application of new optical mapping (BioNano) and extra-long sequencing (PacBio) technologies. Finally, we will establish basal gene expression profiles for major organs and determine the rate of genetic transduction through the organism after external stimulus.