Enhanced Development of the Xiphophorus Model System

剑龙模型系统的增强发展

• 批准号: 9335474
• 负责人: RONALD B WALTER
• 金额: $ 55.05万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9335474
• 关键词: Alleles; Animals; Architecture; Backcrossings; Biological Models; Book Chapters; Book Reviews; Cells; Communities; Complex; Country; Cryopreservation; Data; Databases; Development; Disease; Dissection; Ensure; Experimental Models; Exposure to; Fishes; Gene Expression; Genes; Genetic; Genetic Transcription; Genetic Transduction; Genome; Genomics; Hybrids; Individual; Inheritance Patterns; Journals; Knowledge; Laboratories; Lead; Light; Mind; Modeling; Molecular Profiling; Monoclonal Antibody R24; Monograph; Mutation; Neoplasms; Optics; Organ; Organism; Paper; Peer Review; Phenotype; Predisposition; Principal Investigator; Publications; Recording of previous events; Research; Research Personnel; Resources; Stimulus; System; Technology; Time; United States; Xiphophorus; animal resource; catalyst; design; differential expression; expectation; gene interaction; genetic analysis; genetic pedigree; genome annotation; human disease; improved; melanoma; new technology; novel; programs; sperm cell; symposium; tool; trait; translation to humans; tumor; tumorigenesis

Project Summary This R24 proposal outlines both resource (Aims 1 and 2) and research (Aim 3) components designed to enhance the Xiphophorus Genetic Stock Center (XGSC) that has operated within the United States since the 1930's; making it one of the oldest live animal resource centers worldwide and a national scientific treasure. The XGSC currently houses 58 pedigreed genetic lines representing 24 Xiphophorus species of live-bearing fishes in over 1,400 aquaria. The XGSC has provided pedigreed fish and materials to researchers in over 30 laboratories representing 11 countries. The traditional strength of the Xiphophorus experimental model involves the non-biased assessment of genetic inheritance patterns associated with complex phenotypes. The high genetic variability among Xiphophorus species and capability of producing fertile interspecies hybrids allows inheritance of any observable trait to be followed into individual backcross hybrid progeny. For example, genetic control of tumor susceptibility has been documented in both pure strains and in select Xiphophorus interspecies hybrids, for a variety of spontaneous and induced neoplasms, including several genetically distinct types of melanoma. Proposed improvements to Xiphophorus genomic capabilities promises to support new fundamental knowledge regarding; (a) shifts in the global transcriptional state of cells within the intact organism, (b) complex gene interactions brought about by interspecies hybridization, (c) the genetics underlying melanoma susceptibility, and (d) the initiation and progression of tumorigenesis. Given our preliminary data, we have high expectations for novel scientific findings in the next project period. Specifically, the XGSC will continuing to provide the research community with animals, enhanced resources, and user databases related to the genetically managed lines, and complete cryopreservation of germplasm from all XGSC lines. Enhancements to the genome assemblies will be made by application of new optical mapping (BioNano) and extra-long sequencing (PacBio) technologies. Finally, we will establish basal gene expression profiles for major organs and determine the rate of genetic transduction through the organism after external stimulus.

项目概要 该 R24 提案概述了资源（目标 1 和 2）和研究（目标 3）组成部分，旨在 加强自以来在美国运营的剑尾鱼遗传库存中心 (XGSC) 1930 年代；使其成为世界上最古老的活体动物资源中心之一和国家科学中心 宝藏。 XGSC 目前拥有 58 个纯种遗传系，代表 24 个剑尾鱼物种 1,400 多个水族箱中有活鱼。 XGSC 提供了纯种鱼和材料 代表 11 个国家的 30 多个实验室的研究人员。剑龙的传统实力 实验模型涉及对与相关的遗传模式进行无偏见评估 复杂的表型。剑尾鱼物种之间的高度遗传变异性和生产能力 可育的种间杂交允许将任何可观察到的性状遗传到个体中 回交杂交后代。例如，肿瘤易感性的遗传控制已被记录在 纯品系和精选剑尾属种间杂种，用于各种自发和 诱发肿瘤，包括几种遗传上不同类型的黑色素瘤。 对剑尾鱼基因组能力的拟议改进有望支持新的基础 有关的知识； (a) 完整生物体内细胞整体转录状态的变化，(b) 种间杂交带来的复杂基因相互作用，（c）潜在的遗传学 黑色素瘤易感性，以及（d）肿瘤发生的起始和进展。鉴于我们的初步 数据显示，我们对下一个项目期间的新科学发现抱有很高的期望。具体来说， XGSC 将继续为研究界提供动物、增强的资源和用户 与遗传管理品系相关的数据库，以及完整的种质冷冻保存 所有 XGSC 线路。将通过应用新的光学技术来增强基因组组装 作图（BioNano）和超长测序（PacBio）技术。最后，我们将建立基础 主要器官的基因表达谱并通过 机体受到外界刺激后。