Enhanced Development of the Xiphophorus Model System

剑龙模型系统的增强发展

• 批准号: 9335474
• 负责人: RONALD B WALTER
• 金额: $ 55.05万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9335474
• 关键词: Alleles; Animals; Architecture; Backcrossings; Biological Models; Book Chapters; Book Reviews; Cells; Communities; Complex; Country; Cryopreservation; Data; Databases; Development; Disease; Dissection; Ensure; Experimental Models; Exposure to; Fishes; Gene Expression; Genes; Genetic; Genetic Transcription; Genetic Transduction; Genome; Genomics; Hybrids; Individual; Inheritance Patterns; Journals; Knowledge; Laboratories; Lead; Light; Mind; Modeling; Molecular Profiling; Monoclonal Antibody R24; Monograph; Mutation; Neoplasms; Optics; Organ; Organism; Paper; Peer Review; Phenotype; Predisposition; Principal Investigator; Publications; Recording of previous events; Research; Research Personnel; Resources; Stimulus; System; Technology; Time; United States; Xiphophorus; animal resource; catalyst; design; differential expression; expectation; gene interaction; genetic analysis; genetic pedigree; genome annotation; human disease; improved; melanoma; new technology; novel; programs; sperm cell; symposium; tool; trait; translation to humans; tumor; tumorigenesis

Project Summary This R24 proposal outlines both resource (Aims 1 and 2) and research (Aim 3) components designed to enhance the Xiphophorus Genetic Stock Center (XGSC) that has operated within the United States since the 1930's; making it one of the oldest live animal resource centers worldwide and a national scientific treasure. The XGSC currently houses 58 pedigreed genetic lines representing 24 Xiphophorus species of live-bearing fishes in over 1,400 aquaria. The XGSC has provided pedigreed fish and materials to researchers in over 30 laboratories representing 11 countries. The traditional strength of the Xiphophorus experimental model involves the non-biased assessment of genetic inheritance patterns associated with complex phenotypes. The high genetic variability among Xiphophorus species and capability of producing fertile interspecies hybrids allows inheritance of any observable trait to be followed into individual backcross hybrid progeny. For example, genetic control of tumor susceptibility has been documented in both pure strains and in select Xiphophorus interspecies hybrids, for a variety of spontaneous and induced neoplasms, including several genetically distinct types of melanoma. Proposed improvements to Xiphophorus genomic capabilities promises to support new fundamental knowledge regarding; (a) shifts in the global transcriptional state of cells within the intact organism, (b) complex gene interactions brought about by interspecies hybridization, (c) the genetics underlying melanoma susceptibility, and (d) the initiation and progression of tumorigenesis. Given our preliminary data, we have high expectations for novel scientific findings in the next project period. Specifically, the XGSC will continuing to provide the research community with animals, enhanced resources, and user databases related to the genetically managed lines, and complete cryopreservation of germplasm from all XGSC lines. Enhancements to the genome assemblies will be made by application of new optical mapping (BioNano) and extra-long sequencing (PacBio) technologies. Finally, we will establish basal gene expression profiles for major organs and determine the rate of genetic transduction through the organism after external stimulus.

项目摘要 该R24提案概述了资源（目标1和2）和研究（AIM 3）组件 增强自那以来一直在美国在美国运行的Xiphophorus遗传储备中心（XGSC） 1930年代；使其成为全球最古老的活动物资源中心之一，并且是民族科学的 宝藏。 XGSC目前包含58种典型的遗传线，代表24种Xiphophorus物种 超过1,400个水族箱中的活鱼。 XGSC已为鱼类和材料提供了 代表11个国家的30多个实验室的研究人员。 Xiphophorus的传统力量 实验模型涉及与与遗传遗传模式相关的遗传遗传模式的无偏见评估 复杂的表型。 Xiphophorus物种之间的高遗传变异性和产生能力 肥沃的种间杂种允许遗传任何可观察的性状。 背部杂交后代。例如，已经记录了肿瘤易感性的遗传控制 纯菌株和精选的Xiphophorus inperpecies杂种，用于多种自发和 诱导的肿瘤，包括几种遗传不同类型的黑色素瘤。 Xiphophorus基因组能力的拟议改进有望支持新的基础 关于; （a）完整生物体内细胞的全局转录状态转移，（b） 种间杂交带来的复杂基因相互作用，（c）遗传学的基因相互作用 黑色素瘤的敏感性和（d）肿瘤发生的起始和进展。考虑到我们的初步 数据，我们对下一个项目时期的新科学发现有很高的期望。具体来说， XGSC将继续为研究社区提供动物，增强的资源和用户 与基因管理的线有关的数据库，并从 所有XGSC线。基因组组件的增强功能将通过应用新光学进行 映射（Bionano）和超长测序（PACBIO）技术。最后，我们将建立基础 主要器官的基因表达谱，并确定通过 外部刺激后的生物。