Enhanced Development of the Xiphophorus Model System

剑龙模型系统的增强发展

• 批准号: 7427392
• 负责人: RONALD B WALTER
• 金额: $ 45.01万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7427392
• 关键词: Animal Experimentation; Animal Model; Animals; Appendix; Area; Backcrossings; Behavior; Biological Models; Biological Preservation; Biomedical Research; Breeding; Chromosome Mapping; Communities; Complex; Cryopreservation; Data; Databases; Development; Disease; Ensure; Environment; Evolution; Experimental Animal Model; Experimental Models; Expressed Sequence Tags; Female; Fishes; Freezing; Funding; Future; Genes; Genetic; Genetic Markers; Genetic Recombination; Genome; Genomics; Germany; Human Resources; Hybrids; Individual; Inheritance Patterns; Insemination; Laboratory Research; Letters; Life; Link; Literature; Maps; Mind; Modeling; Molecular; Monoclonal Antibody R24; Numbers; Organism; Phenotype; Physical Map of the Human Genome; Physiology; Procedures; Production; Protocols documentation; Quantitative Trait Loci; Research; Research Personnel; Research Project Grants; Retrieval; Sampling; Scientist; Source; Specimen; Sperm Motility; Spermatogonia; Standards of Weights and Measures; Synteny; System; Taxon; Testing; Texas; United States; Vertebrates; Xiphophorus; Yang; austin; catalyst; cohort; day; design; expectation; experience; genetic analysis; interest; life history; male; physical mapping; professor; programs; sperm cell; technology development; tool; trait

DESCRIPTION (provided by applicant): Xiphophorus fishes have been used as an experimental model for fundamental biomedical research for over 75 years. Eventually "mechanistic" hypothesis must be confirmed using intact animal model systems. Well-characterized diverse experimental animal models are best suited to provide this function. In our future scientific environment, diverse animal models that allow us to better understand complex heritable (multigenic) traits will be of considerable importance as we attempt to unravel the genetics underlying complex traits such as behavior and the components leading to alternative in life histories. This R24 proposal is designed to maintain and enhance the Xiphophorus Genetic Stock Center (XGSC). The XGSC has operated within the United States since the 1930's. The traditional strength of the Xiphophorus model involves the non-biased assessment of genetic inheritance patterns associated with complex (multi-genetic) phenotypes within intact animals. The extreme genetic variability among Xiphophorus species (26 known taxa) and capability of producing fertile interspecies hybrids allows chromosomal/marker inheritance to be followed in individual backcross hybrid progeny. Statistical analyses involving hundreds of animals in a cohort can be used to identify markers linked to any trait of interest, so long as the phenotype (as a singular trait or QTL) can be discerned between two parental lines/species and in hybrid progeny. This experimental system is increasingly useful to test hypotheses generated from analyses of whole animal genomes (see 39+ letters of support for the XGSC, appended). With these expectations in mind, it is reasonable to continue to provide pedigreed Xiphophorus fish and associated materials to research laboratories, to continue our progress in development of cryopreservation procedures to ensure long term availability of stocks, and to further develop contemporary genomic tools for research scientists using and/or anticipating use of this valuable vertebrate experimental model. Accordingly our specific aims are: (1) To preserve and enhance the Xiphophorus Genetic Stock Center and provide the research community with animals and materials from genetically managed lines according to the specifications of each investigator. (2) To develop an extensive database of expressed sequence tag (EST) markers. EST marker annotation and the databases will be made available to the community. (3) We will collate the Xiphophorus recombination gene map developed in the previous project period with the BAC minimal tiling path (BAC-MTP) physical map currently being constructed. (4) To continue development of technology allowing cryopreservation of Xiphophorus sperm samples and to provide a preservation program where the XGSC fish lines are maintained as cryopreserved specimens to protect their genetic heritage.

描述(申请人提供)：剑鱼作为基础生物医学研究的实验模型已有超过75年的历史。最终，“机械论”假说必须用完整的动物模型系统来证实。具有良好特性的各种实验动物模型最适合提供这种功能。在我们未来的科学环境中，使我们能够更好地理解复杂的可遗传(多基因)特征的多样化的动物模型将具有相当重要的意义，因为我们试图揭开复杂特征的遗传学基础，如行为和导致生活史上另类选择的成分。这份R24提案旨在维护和加强剑鱼遗传资源中心(XGSC)。XGSC自20世纪30年代的S以来一直在美国境内运作。剑鱼模型的传统优势包括对与完整动物的复杂(多基因)表型相关的遗传遗传模式进行无偏见的评估。剑尾鱼物种(26个已知类群)之间的极端遗传变异和产生可育的种间杂交的能力使得染色体/标记遗传可以在个体回交杂交后代中进行。一个队列中涉及数百只动物的统计分析可以用来识别与任何感兴趣的性状相关联的标记，只要能在两个亲本系/种之间和杂交后代中辨别出表型(作为单一性状或QTL)。这一实验系统对检验从动物全基因组分析中产生的假说越来越有用(见39多封支持XGSC的信件，附后)。考虑到这些期望，有理由继续向研究实验室提供纯种剑尾鱼和相关材料，继续我们在冷冻程序开发方面的进展以确保种群的长期可用性，并为使用和/或预期使用这一宝贵的脊椎动物实验模型的研究科学家进一步开发当代基因组工具。因此，我们的具体目标是：(1)保护和加强剑鱼遗传资源中心，并根据每位研究人员的具体要求，向研究界提供来自基因管理品系的动物和材料。(2)建立表达序列标签(EST)标记数据库。EST标记注释和数据库将向社区提供。(3)将前期开发的剑鱼重组基因图谱与目前正在构建的BAC最小平铺路径(BAC-MTP)物理图谱进行比对。(4)继续开发允许超低温保存剑鱼精子样本的技术，并提供一个保存计划，将XGSC鱼线作为超低温保存的样本进行保存，以保护其遗传遗产。