Core--Microscopy, Image Analysis and Stereology

核心——显微镜、图像分析和体视学

• 批准号: 7060633
• 负责人: Patrick G Sullivan
• 金额: $ 12.24万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7060633
• 关键词: bioimaging /biomedical imaging; biomedical facility; brain injury; digital imaging; fluorescence microscopy; image processing; microscopy; neuroimaging; spinal cord injury; video microscopy

As proposed, the Microscopy, Image Analysis & Stereology Core would be created as an independent core completely separated from the existing Microscopy Facility at this institution. The core would, however, be equipped with current in-house equipment made available by SCoBIRC faculty including two Olympus Provis AX70 Fluorescence Microscopes (Image Pro interfaced via MagnaFire Digital Camera), Olympus BX 41 with Video Camera, Olympus BX 50 Epifluorescent Microscope (Bioquant Stereology package + 3D topographer interfaced with MagnaFire Digital Camera & Optronics Video Camera), Nikon E400 teaching microscopy with DAGE Video Camera. Specifically, the proposed Microscopy, Image Analysis & Stereology Core will combine the existing equipment, which is currently scattered among various NINDS-funded investigators' laboratories, with the additional new equipment and dedicated technical oversight. It would also place all these facilities under one administrative structure. Accordingly, the proposed core requests the provision of a senior technician who will oversee the use of the image analysis and stereology equipment as well as the desired confocal microscope.

按照提议，显微镜、图像分析和立体学核心将作为一个独立的核心，与该机构现有的显微镜设施完全分离。然而，核心将配备由SCoBIRC教师提供的现有内部设备，包括两台奥林巴斯Provis AX70荧光显微镜（通过MagnaFire数码相机接口的Image Pro），奥林巴斯BX 41带摄像机，奥林巴斯BX 50 epifluorescence显微镜（Bioquant立体学包+ 3D地形仪与MagnaFire数码相机和Optronics摄像机接口），尼康E400教学显微镜与DAGE摄像机。具体来说，拟议的显微镜、图像分析和立体学核心将把目前分散在ninds资助的各个研究人员实验室中的现有设备与额外的新设备和专门的技术监督结合起来。它还将把所有这些设施置于一个行政结构之下。因此，拟议的核心要求提供一名高级技术人员，负责监督图像分析和立体学设备以及所需的共聚焦显微镜的使用。