MICROSCOPY CORE

显微镜、图像分析和体视学中心位于BBSRB四楼，提供神经创伤和其他神经科学研究所需的先进仪器，包括两台Olympus Provis AX70荧光显微镜（Image Pro通过MagnaFire数码相机接口），奥林巴斯BX 41摄像机，Olympus BX 50落射荧光显微镜（Bioquant Stereology包+与MagnaFire数码相机和Optronics摄像机连接的3D地形图仪）， Zeiss Axiovert 200 M显微镜和带DAGE摄像机的Nikon E400教学显微镜。除了现有的设备外，SCoBIRC最近还在NIH的资助下投资了三台新的专业显微镜。第一台是奥林巴斯FluoViewTM 300点扫描、点检测、共聚焦激光扫描显微镜，专为生物学研究应用而设计，具有出色的分辨率、 激励和直观的用户友好界面，包括为个人用户预设的参数。FluoViewTM 300还能够进行3D渲染和延时观察，并可生成TTL I/O信号，以协调与外部仪器的实验时间。此外，两个高灵敏度光电倍增管（PMT）直接位于FV 300共焦荧光发射光路内，用于高灵敏度检测荧光信号。第二个体视显微镜是倒置的 Olympus IX 81配备了电动载物台编码器和BioQuant Nova Prime软件，特别适合使用现代体视学工具进行定量。核心由核心主管帕特里克沙利文博士担任，SCoBIRC副主任，解剖学和神经生物学副教授和助理主任，亚历山大Rabchevsky，博士，生理学副教授，他们都很好 精通使用多重图像分析终点测量和共焦成像作为他们研究的终点。两人都在开发用于评估神经创伤研究中组织保留和细胞损失的协议方面拥有专业知识。

The Microscopy, Image Analysis and Stereology Core, located on the BBSRB fourth floor, provides advanced instrumentation required for neurotrauma and other neuroscience research, including two Olympus Provis AX70 Fluorescence Microscopes (Image Pro interfaced via MagnaFire Digital Camera), an Olympus BX 41 with Video Camera, an Olympus BX 50 Epifluorescent Microscope (Bioquant Stereology package + 3D topographer interfaced with MagnaFire Digital Camera and Optronics Video Camera), a Zeiss Axiovert 200 M microscope, and a Nikon E400 teaching microscope with DAGE Video Camera. In addition to this existing equipment, SCoBIRC recently invested in three new specialized microscopes with funding from the NIH. The first, an Olympus FluoViewTM 300 point-scanning, point-detection, confocal laser scanning microscope designed for biology research applications, has excellent resolution, efficiency of excitation, and an intuitive, user-friendly interface including preset parameters for individual users. The FluoViewTM 300 is also capable of 3D rendering and time-lapse observation, and TTL I/O signals can be generated to coordinate experiments timed with external instrumentation. Additionally, two high-sensitivity photomultiplier tubes (PMTs) are located directly within the FV300 confocal fluorescence emission light path for high sensitivity detection of the fluorescence signal. The second stereology microscope is an inverted Olympus IX 81 outfitted with motorized stage encoders and the BioQuant Nova Prime software, particulariy well suited for quantification using modern stereological tools. The core is staffed by a Core Director, Patrick Sullivan, Ph.D., SCoBIRC Associate Director and Associate Professor of Anatomy & Neurobiology and an Assistant Director, Alexander Rabchevsky, Ph.D., Associate Professor of Physiology both of whom are well versed in the use of multiple image analysis endpoint measures and confocal imaging as endpoints in theirresearch. Both have expertise in developing protocols used to assess tissue sparing and cell loss in neurotrauma research.