CORE-- Neuroanatomy-Imaging

应审查小组的要求，已从神经解剖成像核心中删除了定量光学显微镜(LM)密度测量的使用。此外，还进行了修改，以反映项目建议书的变化和删除项目4。具体而言，增加了光镜和电子显微镜放射自显影、Dia顺行追踪和TUNEL标记技术，删除了细胞旁标记、生物细胞素顺行追踪和光镜重建技术。抗血清中也有详细的变化：对抗孕酮受体α2a-肾上腺素能的抗血清的描述 现在包括受体和p22-Phox，而针对5HT受体的抗血清、c-Fos、nNOS和加压素已被删除。反映删除项目4的项目对神经解剖成像核心的利用百分比也发生了变化。对核心的订正说明如下。主要变化由左边空白处的一条线表示。总体而言，预算已经减少。预算变动的详细说明已列入预算理由。

As requested by the review team, the use of quantitative light microscopic (LM) densitometry has been removed from the Neuroanatomy-lmaging Core. In addition, changes have been made that reflect changes in the project proposals and the deletion of Project 4. Specifically, LM and EM autoradiography, DiA anterograde tract-tracing, and TUNEL labeling techniques have been added, and juxtacellular labeling, biocytin anterograde tract-tracing, and LM reconstruction techniques have been deleted. There also have been changes in the antisera that are detailed: descriptions of antisera raised against the progesterone receptor, the alpha2A-adrenergic receptor and p22-phox are now included, while antisera raised against 5HT receptors, c-Fos, nNOS and vasopressin have been deleted. There have also been changes in the percent utilization of the Neuroanatomy-lmaging Core by the projects reflecting the deletion of Project 4. The revised description of the core is provided below. Major changes are indicated by a line in the left margin. Overall, the Budget has been reduced. A detailed account of the budgetary changes has been included in the Budget Justification.