Core--ES Cell and Microinjection Facility

本项目中关于早期心脏发育遗传学的组成部分研究将技术和生物学重点放在作为研究对象的小鼠身上。在脊椎动物中，只有老鼠允许如此广泛的基因操作。这些项目包括通过在胚胎干细胞中进行同源重组或在体内进行Cre/IoxP重组，在小鼠的生殖系和体细胞组织中产生突变。其他需求还包括培育携带细菌人工染色体（BAC）的小鼠、常规转基因、可诱导转基因或将工程细胞引入囊胚。这些操作胚胎干细胞和构建小鼠的程序在技术上要求很高，并且在专门的核心实验室中最有效地进行。所有这些等位基因和技术都已由核心主任成功实施，许多具体创新都是通过本项目先前的支持开发的。

The component studies in this Program Project on the genetics of early cardiac development share a technical and biological focus on the mouse as the organism studied. Among vertebrates, only the mouse permits so robust a range of genetic manipulations. The projects involve creating mutations in the germ line and somatic tissues of mice by performing homologous recombination in ES cells, or Cre/IoxP recombination in vivo. Other needs include the creation of mice harboring a bacterial artificial chromosome (BAC), conventional transgene, inducible transgene, or engineered cells that are introduced into blastocysts. These procedures for manipulation of ES cells and the construction of mice are technically demanding and most efficiently performed in a dedicated core laboratory. All of these alleles and technologies have been successfully implemented by the Core Directors, and many of the specific innovations were developed through prior support of this Project.