Core--ES Cell and Microinjection Facility

该计划项目中关于早期心脏发育遗传学的组成部分研究，在生物体研究的同时，共享对小鼠的技术和生物学重点。在脊椎动物中，只有老鼠允许如此强大的一系列基因操作。这些项目包括通过在ES细胞中进行同源重组，或在体内进行Cre/IoxP重组，在小鼠的生殖系和躯体组织中创造突变。其他需求包括创造携带细菌人工染色体(BAC)、常规转基因、可诱导转基因或工程细胞的小鼠，这些细胞被引入囊胚。这些操作胚胎干细胞和构建小鼠的程序在技术上要求很高，并且在专门的核心实验室中进行得最有效。所有这些等位基因和技术都已由核心董事成功实施，许多具体的创新都是通过该项目的先前支持而开发的。

The component studies in this Program Project on the genetics of early cardiac development share a technical and biological focus on the mouse as the organism studied. Among vertebrates, only the mouse permits so robust a range of genetic manipulations. The projects involve creating mutations in the germ line and somatic tissues of mice by performing homologous recombination in ES cells, or Cre/IoxP recombination in vivo. Other needs include the creation of mice harboring a bacterial artificial chromosome (BAC), conventional transgene, inducible transgene, or engineered cells that are introduced into blastocysts. These procedures for manipulation of ES cells and the construction of mice are technically demanding and most efficiently performed in a dedicated core laboratory. All of these alleles and technologies have been successfully implemented by the Core Directors, and many of the specific innovations were developed through prior support of this Project.