Nano mirrors for high throughput cell motion analysis

用于高通量细胞运动分析的纳米镜

• 批准号: 6941923
• 负责人: James K Gimzewski
• 金额: $ 18.74万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-15 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6941923
• 关键词: cell membrane; cell motility; fiber optic microscopy; high throughput technology; interferometry; lasers; nanotechnology; technology /technique development

DESCRIPTION (provided by applicant): This grant proposal is in response to specific NIH PAR-03-045 'Nanoscience and Nanotechnology in Biology and Medicine' and utilizes the R21 mechanism. We will extend proof-of-concept nanomechanical cell studies conducted by the Gimzewski & Teitell labs. Using atomic force microscopy (AFM), we have documented novel, treatment- and strain-dependent motion signatures from resting and stimulated yeast cells and have conducted similar but more preliminary measurements in mammalian cells. Importantly, initial experiments indicate that these cell-specific mechanical signals yield real-time diagnostic information about cell structure, metabolism and movement, along with response to chemical and physical stimuli. We specifically seek support to characterize further the nanomechanical features of mammalian cells and to develop a higher-throughput experimental approach. Our new approach will make use of "nano mirrors" fixed to the cell membrane, in place of the AFM tip. These mirrors act as nanoscopic displacement probes and can be interrogated by laser scanning metrology (using an optical lever or a fiber-optic interferometer). This configuration is parallel, enabling simultaneous nanomechanical measurements of hundreds-to- thousands of individual cells. We will document the ability our nano mirror concept to provide information about a cell's state of health and functioning, in a variety of environmental conditions.

描述（由申请人提供）：该赠款提案是针对特定的NIH PAR-03-045“生物学和医学中的纳米科学和纳米技术”，并利用R21机制。我们将延长Gimzewski＆Teitell Labs进行的概念验证纳米力学细胞研究。使用原子力显微镜（AFM），我们记录了来自静息和刺激酵母细胞的新型，治疗和应变依赖性运动信号，并在哺乳动物细胞中进行了相似但更初步的测量。重要的是，初始实验表明，这些细胞特异性的机械信号产生了有关细胞结构，代谢和运动以及对化学和物理刺激的反应的实时诊断信息。我们特别寻求支持，以进一步表征哺乳动物细胞的纳米力学特征，并开发出更高的实验方法。我们的新方法将利用固定在细胞膜上的“纳米镜”代替AFM尖端。这些镜子充当纳米镜位移探针，可以通过激光扫描计量学询问（使用光杆或光纤干涉仪）。这种配置是平行的，可以同时对数千个单个单个单元格进行同时进行纳米力学测量。我们将记录我们的纳米镜像概念在各种环境条件下提供有关细胞健康状况和功能状态的信息。