Analysis of the Sensory Motion of MicroOrganisms

微生物的感觉运动分析

• 批准号: 6736592
• 负责人: SHAHID M KHAN
• 金额: $ 10.7万
• 依托单位: MOTION ANALYSIS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-19 至 2006-03-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6736592
• 关键词: bioimaging /biomedical imaging; biological signal transduction; cell motility; cilium /flagellum motility; computer program /software; computer system design /evaluation; digital imaging; fiber optic microscopy; microorganism; microorganism interaction; microorganism population study; sensory mechanism

DESCRIPTION (provided by applicant): The motile behavior of microorganisms is based on the sensory transduction pathways that provide important design principles for the intracellular signal relays in living cells. Motion Analysis Inc. (MA) has been a leader in building commercial motion analysis systems for many decades. Over the past decade, the company has incorporated important advances in camera imaging and computer software into its 3D systems for analysis of macroscopic movement such as human gait. During this period, understanding of sensory transduction has advanced to a stage where refined quantitative analysis of the motion has become essential for the quantitative modeling of signal pathways. In addition, optical probes for monitoring intermediate signal processing reactions have become available. We plan to develop a spatially and temporally resolved system for high-throughput quantitative analysis of the motion of micro-organisms by coupling the expertise gained by Motion Analysis over the past decade with biotechnological progress. For this purpose, a research team based primarily at the Molecular Biology Consortium (MBC) has been assembled with strong research expertise in key problem areas, microscopic imaging and analysis. The combined MA/MBC team will utilize the initial phase I of the award to mate MA's digital camera technology optimally to the optical microscope and develop graphical user interface software that is both flexible and expansion capable. Once this is done and optimal microscope configuration has been established, we will be able to formulate clear strategies for camera design and software operator expansion needed for imaging the biochemical signaling state of the micro-organisms simultaneously with shape and motion analysis. The end output of this effort should be system that will capture a small but established market of well-funded research teams. In addition, the increased sophistication and the simultaneous measurement capability of mechanical together with chemical parameters down to the single cell level should open up scenarios where the possible use of this system in medical diagnostics and environmental science applications may be envisaged, based on the biosensor potential of the chosen microbes.

描述（由申请人提供）：微生物的运动行为基于感官转导途径，这些途径为活细胞中细胞内信号中继提供了重要的设计原理。 Motion Analysis Inc.（MA）已成为建立商业运动分析系统的领导者数十年。在过去的十年中，该公司将相机成像和计算机软件中的重要进展纳入其3D系统中，以分析诸如人体步态之类的宏观运动。在此期间，对感觉转导的理解已发展到一个阶段，在该阶段，对运动的精致定量分析对于信号途径的定量建模至关重要。另外，用于监测中间信号处理反应的光学探针已获得。我们计划开发一个在空间和时间上解决的系统，以通过将过去十年中的运动分析与生物技术进步相连，以对微生物的运动进行高通量定量分析。为此，主要基于分子生物联盟（MBC）的研究团队已在关键问题领域，微观成像和分析方面具有强大的研究专业知识。合并后的MA/MBC团队将利用该奖项的初始阶段I阶段，以最佳的方式将MA的数码相机技术与光学显微镜相配，并开发具有灵活性和扩展功能的图形用户界面软件。完成此操作并建立了最佳显微镜配置后，我们将能够为相机设计和软件操作员扩展制定清晰的策略，以通过形状和运动分析同时成像微生物的生化信号传导状态。这项工作的最终产出应该是系统，该系统将捕捉一个庞大但已建立的资金资助的研究团队的市场。此外，机械参数以及至单细胞水平的化学参数的同时成熟和同时测量能力应打开场景，在这些情况下，可以基于Chose Microbes的生物传感器潜力来设想该系统在医学诊断和环境科学应用中可能使用该系统。