RNA Transport Within the Nucleus

细胞核内的 RNA 运输

• 批准号: 6944024
• 负责人: THORU PEDERSON
• 金额: $ 25.44万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6944024
• 关键词: RNA binding protein; cell nucleus; fluorescence microscopy; fluorescent dye /probe; globin; guanosinetriphosphatases; in situ hybridization; intracellular transport; messenger RNA; microinjections; nucleic acid quantitation /detection; oligonucleotides; protooncogene; ribosomal RNA; tissue /cell culture; transcription factor

DESCRIPTION (provided by applicant): This research project involves a team of two cell biologists and a biophysicist who have productively collaborated recently and now are joining together to undertake studies of RNA localization and traffic in the cell nucleus. The proposed research is virtually unique in that intranuclear RNA movements are tracked in living cells, rather than by localization methods that require chemical fixation. The importance of this approach cannot be overstated, since it opens the door to the in vivo investigation of dynamic pathways of intranuclear RNA transport. The two major experimental strategies to be taken, both developed in the P.I?s laboratory, are the fluorescent tagging of endogenous nuclear RNAs and the microinjection of fluorescent RNAs into mammalian cell nuclei. A diffusion-based model for nuclear RNA transport has emerged from this initial work and this will be further investigated. The proposed studies will characterize in detail the intranuclear trafficking patterns of ribosomal RNA as well as beta-globin and c-fos mRNAs. Mutant beta-globin RNAs will be investigated to relate processing defects to intranuclear transport and localization. Studies will also be undertaken of the spliced and polyadenylated but nucleus-restricted RNA, Xist, which is an unusual transcript produced by the inactive X chromosome and is predicted to display very different intranuclear transport properties than mRNAs. Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP.

描述（由申请人提供）：本研究项目涉及一个团队

项目成果

In search of nonribosomal nucleolar protein function and regulation.

• DOI: 10.1083/jcb.200812014
• 发表时间: 2009-03-23
• 期刊: The Journal of cell biology
• 作者: Pederson T;Tsai RY
• 通讯作者: Tsai RY

The spatial organization of the genome in mammalian cells.

• DOI: 10.1016/j.gde.2004.02.008
• 发表时间: 2004-04
• 期刊: Current opinion in genetics & development
• 影响因子: 4
• 作者: T. Pederson

Author, author burning bright; author, author are you right?

作者，作家，燃烧着光明；

• DOI: 10.1096/fj.06-0405ufm
• 发表时间: 2006
• 期刊: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
• 作者: Pederson,Thoru

50 years ago protein synthesis met molecular biology: the discoveries of amino acid activation and transfer RNA.

50 年前，蛋白质合成遇到了分子生物学：氨基酸激活和转移 RNA 的发现。

• DOI: 10.1096/fj.05-1002ufm
• 发表时间: 2005
• 期刊: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
• 作者: Pederson,Thoru

The sea urchin's siren.

海胆的海妖。

• DOI: 10.1016/j.ydbio.2006.10.006
• 发表时间: 2006
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Pederson,Thoru