Photonic crystal technology for biomolecular assay

用于生物分子测定的光子晶体技术

• 批准号: 6961254
• 负责人: THEODORE B NORRIS
• 金额: $ 18.8万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-21 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6961254
• 关键词: bioassay; biomedical equipment development; biosensor device; chemical binding; chemical kinetics; computer data analysis; computer program /software; computer simulation; computer system design /evaluation; intermolecular interaction; mathematical model; photonics; technology /technique development

DESCRIPTION (provided by applicant): A novel technique based on photonic crystals (PC) will be developed and used to construct an extremely sensitive instrument for the study of biomolecular affinities and binding kinetics. We have previously demonstrated that a narrow midgap transmission peak can be formed and a local field enhancement can be achieved when a defect layer is sandwiched between two PCs. In this R21 application, we will explore for the first time the possibility to use only one PC in a total internal reflection geometry, so that the defect layer becomes accessible for binding of analyte molecules. When the defect layer is doped with absorbing materials, the spectrum of the reflected light from this PC structure will have a very narrow but prominent dip due to the enhanced absorption in the defect layer. The dip wavelength will depend very sensitively on the thickness and/or refractive index of the biomolecules bound to the defect layer. This will enable highly sensitive measurements of the binding of biomolecules and the kinetics of this process. We will focus on developing this novel methodology and constructing a highly sensitive PC based instrument. First, we will optimize our design of the PC structure based on comprehensive theoretical modeling and numerical simulations in order to achieve the maximum detection sensitivity. Then we will construct a working system using the optimized PC structure as a critical component. We will also explore several possible detection modes and develop related software for data analysis and interpretation. Finally, this instrument will be tested using several biological model systems in order to demonstrate its applicability. This new instrument will possess a number of advantages over the currently widely used surface plasmon resonance (SPR) based instrument and other waveguide based instruments. It will have the capability to study, in a quantitative manner and without requirement of labeling, many different dynamic molecular interactions in real time, allowing affinity measurements, concentration determinations of specific analytes, and kinetic analyses. We will compare directly the sensitivity of our technique to that of commercially available state-of- the-art SPR-based systems. CRITIQIUE

描述（由申请人提供）： 将开发一种基于光子晶体（PC）的新技术，并用于构建极其灵敏的仪器，用于研究生物分子亲和力和结合动力学。我们之前已经证明，当缺陷层夹在两个 PC 之间时，可以形成窄的中带隙传输峰并实现局部场增强。在此 R21 应用中，我们将首次探索在全内反射几何结构中仅使用一个 PC 的可能性，以便缺陷层易于与分析物分子结合。当缺陷层掺杂吸收材料时，由于缺陷层的吸收增强，来自该PC结构的反射光的光谱将具有非常窄但显着的下降。浸入波长将非常敏感地取决于与缺陷层结合的生物分子的厚度和/或折射率。这将使生物分子的结合和该过程的动力学进行高度灵敏的测量。 我们将专注于开发这种新颖的方法并构建高度灵敏的基于 PC 的仪器。首先，我们将基于综合理论建模和数值模拟来优化PC结构设计，以实现最大的检测灵敏度。然后我们将以优化的PC结构作为关键组件构建一个工作系统。我们还将探索几种可能的检测模式并开发相关软件进行数据分析和解释。最后，该仪器将使用多个生物模型系统进行测试，以证明其适用性。与目前广泛使用的基于表面等离子体共振（SPR）的仪器和其他基于波导的仪器相比，这种新仪器具有许多优点。它将能够以定量方式且无需标记地实时研究许多不同的动态分子相互作用，从而允许亲和力测量、特定分析物的浓度测定和动力学分析。我们将直接将我们的技术的灵敏度与市售的最先进的基于 SPR 的系统进行比较。 批评