Photonic crystal technology for biomolecular assay

用于生物分子测定的光子晶体技术

• 批准号: 7251887
• 负责人: THEODORE B NORRIS
• 金额: $ 17.93万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-21 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7251887
• 关键词: Address; Affinity; Antibodies; Antigens; Arts; Binding; Biological; Biological Assay; Biological Models; Cell physiology; Chemicals; Computer software; Concentration measurement; DNA-Protein Interaction; Data Analyses; Defect; Dependence; Detection; Equipment; Film; Foundations; Kinetics; Label; Ligands; Light; Lipids; Measurement; Measures; Metals; Methodology; Methods; Numbers; Oligonucleotides; Oligosaccharides; Optics; Process; Proteins; Refractive Indices; Reporting; Research Personnel; Structure; Surface; Surface Plasmon Resonance; System; Techniques; Technology; Testing; Theoretical model; Thick; Time; Width; Work; absorption; base; design; improved; instrument; macromolecule; novel; photonics; programs; response; simulation; transmission process

A novel technique based on photonic crystals (PC) will be developed and used to construct an extremely sensitive instrument for the study of biomolecular affinities and binding kinetics. We have previously demonstrated that a narrow midgap transmission peak can be formed and a local field enhancement can be achieved when a defect layer is sandwiched between two PCs. In this R21 application, we will explore for the first time the possibility to use only one PC in a total internal reflection geometry, so that the defect layer becomes accessible for binding of analyte molecules. When the defect layer is doped with absorbing materials, the spectrum of the reflected light from this PC structure will have a very narrow but prominent dip due to the enhanced absorption in the defect layer. The dip wavelength will depend very sensitively on the thickness and/or refractive index of the biomolecules bound to the defect layer. This will enable highly sensitive measurements of the binding of biomolecules and the kinetics of this process. We will focus on developing this novel methodologyand constructing a highly sensitive PC based instrument. First, we will optimize our design of the PC structure based on comprehensive theoretical modeling and numerical simulations in order to achieve the maximum detection sensitivity. Then we will construct a working system using the optimized PC structure as a critical component. We will also explore several possible detection modes and develop related software for data analysis and interpretation. Finally, this instrumentwill be tested using several biological model systems in order to demonstrate its applicability. This new instrument will possess a number of advantages over the currently widely used surface plasmon resonance (SPR) based instrument and other waveguide based instruments. It will have the capability to study, in a quantitative manner and withoutrequirement of labeling, many different dynamic molecular interactions in real time, allowing affinity measurements, concentration determinations of specific analytes, and kinetic analyses. We will compare directly the sensitivity of our technique to that of commercially available state- of-the-art SPR-based systems.

一种基于光子晶体（PC）的新技术将被开发并用于构建一个非常敏感的 用于研究生物分子亲和性和结合动力学的仪器。我们以前已经证明，一个狭窄的 当缺陷层中 夹在两台PC之间。在此R21应用程序中，我们将首次探索仅使用一个 PC在全内反射几何结构中，使得缺陷层变得可用于分析物分子的结合。 当缺陷层掺杂有吸收材料时，来自该PC结构的反射光的光谱将 由于在缺陷层中的增强的吸收而具有非常窄但显著的下降。倾角波长将 非常敏感地取决于与缺陷层结合的生物分子的厚度和/或折射率。这将 能够高灵敏度地测量生物分子的结合和该过程的动力学。 我们将致力于开发这种新的方法学和构建一个高灵敏度的基于PC的仪器。第一、 我们将根据全面的理论建模和数值计算， 模拟，以实现最大的检测灵敏度。然后，我们将使用 优化的PC结构作为关键部件。我们还将探索几种可能的检测模式，并开发 相关软件进行数据分析和解释。最后，该仪器可以使用几种生物 模型系统，以证明其适用性。这一新工具将具有许多优点， 目前广泛使用的基于表面等离子体共振（SPR）的仪器和其它基于波导的仪器。它 将有能力研究，在定量的方式和没有标签的要求，许多不同的动态 真实的分子相互作用，允许亲和性测量，特定分析物的浓度测定， 和动力学分析。我们将直接比较我们技术的灵敏度与商业上可用的状态- 最先进的SPR系统

项目成果

Label-free biosensing using a photonic crystal structure in a total-internal-reflection geometry.

• DOI: 10.1117/12.808369
• 发表时间: 2009-02-16
• 期刊: Proceedings of SPIE--the International Society for Optical Engineering
• 作者: Guo Y;Ye JY;Divin C;Thomas TP;Myc A;Bersano-Begey TF;Baker JR;Norris TB
• 通讯作者: Norris TB

Optical fiber-based in vivo quantification of growth factor receptors.

基于光纤的生长因子受体体内定量。

• DOI: 10.1002/cncr.26487
• 发表时间: 2012
• 期刊: Cancer
• 影响因子: 6.2
• 作者: Thomas,ThommeyP;Chang,Yu-Chung;Ye,JingYong;Kotlyar,Alina;Cao,Zhengyi;Shukla,Rameshwer;Qin,Suyang;Norris,TheodoreB;BakerJr,JamesR
• 通讯作者: BakerJr,JamesR

Real-time biomolecular binding detection using a sensitive photonic crystal biosensor.

• DOI: 10.1021/ac100576y
• 发表时间: 2010-06-15
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Guo, Yunbo;Ye, Jing Yong;Divin, Charles;Huang, Baohua;Thomas, Thommey P.;Baker, James R., Jr.;Norris, Theodore B.
• 通讯作者: Norris, Theodore B.

Folate-targeted nanoparticles show efficacy in the treatment of inflammatory arthritis.

• DOI: 10.1002/art.30459
• 发表时间: 2011-09
• 期刊: ARTHRITIS AND RHEUMATISM
• 作者: Thomas, Thommey P.;Goonewardena, Sascha N.;Majoros, Istvan J.;Kotlyar, Alina;Cao, Zhengyi;Leroueil, Pascale R.;Baker, James R., Jr.
• 通讯作者: Baker, James R., Jr.

Sensitive molecular binding assay using a photonic crystal structure in total internal reflection.

• DOI: 10.1364/oe.16.011741
• 发表时间: 2008-08-04
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Guo Y;Divin C;Myc A;Terry FL Jr;Baker JR Jr;Norris TB;Ye JY
• 通讯作者: Ye JY