Intracellular Infection by Invasive Group A Streptococci

EXCEED提供的空间。该项目将确定A组链球菌的细胞内侵袭和致病机制之间的关系。实验将进一步研究链球菌侵占宿主细胞外基质(ECM)和受体信号系统以达到细胞内状态的分子机制。体内实验将采用氨基酸鼻腔感染模型，探讨细胞内侵袭在鼻腔相关淋巴组织持续感染中的作用。实验将评估链球菌是否优先感染这一组织。我们认为，持久性取决于链球菌被淋巴组织中特定细胞有效摄取的潜力。从这项研究中获得的信息可能会确定抗生素开发的新靶点，并增加我们对这种病原体在人类群体中持续存在的机制的理解。演出现场(S)(组织，城市，州)美国明尼苏达大学明尼阿波利斯分校主要人员。请参阅第11页的说明。根据需要使用续页，以下面所示的格式提供所需信息。名称组织在明尼苏达大学保罗·帕特里克·克莱里项目中的作用。明尼苏达大学研究员Mae-Sun Park博士后培训生！&GT；明尼苏达大学普鲁绍塔曼大学博士后培训生T‘hs 398(版本4/98)在整个申请过程中，第2页BB页码连续显示在底部。不要使用3a、3b等后缀。========================================Section End===========================================

EXCEEDTHE SPACE PROVIDED. This project will define the relationship betweenintracellular invasion andgroup A streptococcal pathogenesis. Experiments willfurther investigate the molecular mechanisms by which streptococci commandeerthe extracellular matrix (ECM)and receptor signaling systems of host colls to reachan intracellular state. Invivo experimentswill investigate the role of intracellular invasion in persistent infection of nasal associatedlymphoid tissue, employing amurine intranasal infection model. Experiments willevaluate whether streptococci preferentially infect this tissue. Wepropose that persistence depends onthe potentialof streptococci to beefficiently ingested by specific cells in this lymphoid tissue. Information gainedfromthis studymay identify newtargets for antibiotic development,and increaseour understanding of the mechanismsby which this pathogen persists in human populations. PERFORMANCE SITE(S) (organization, cily, state) University of Minnesota - Minneapolis MN, USA KEY PERSONNEL. See instructions on Page 11. Use continuation pages as needed to provide the required information in the format shownbelow. Name Organization Role on Project Paul Patrick Cleary University of Minnesota Prin. Investigator Mae-Sun Park University of Minnesota PostdoctoralTrainee !>ai Sudha Purushothaman University of Minnesota PostdoctoralTrainee T'HS 398 (Rev. 4/98) Page 2 BB Number pages consecutively at the bottom throughout the application. Do not use suffixes such as 3a, 3b. ========================================Section End===========================================