T cell immune response to group A streptococci in NALT

NALT 中 A 族链球菌的 T 细胞免疫反应

• 批准号: 6867089
• 负责人: PAUL Patrick CLEARY
• 金额: $ 29.41万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-15 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6867089
• 关键词: Streptococcus infection; Streptococcus pyogenes; T lymphocyte; antigen antibody reaction; bacteria infection mechanism; cellular immunity; delayed hypersensitivity; genetically modified animals; helper T lymphocyte; immune response; inhalation drug administration; laboratory mouse; lymphatic tissue; membrane proteins; ovalbumin; passive immunization; peptidases; pharyngitis

DESCRIPTION (provided by applicant): Five million people are annually diagnosed with streptococcal pharyngitis, and 1200-1500 die annually from complications of group A streptococcal (GAS) infections in the United States alone. The over all goal of this projection is to understand the cellular and immunological reason that up to a third of children vigorously treated for pharyngitis continue to shed streptococci and why even more fail to develop a measurable immune response. Our in investigation will make use of a well-developed intranasal infection murine model to mimic infection of human tonsils. Preliminary results show that GAS have an efficient tropism for lymphoid tissue (NALT) beneath nasal passages of BalBc. Adoptive transfer of transgenic, naive ovalbumin specific CD4 T cells will be used to study the T cell based immune response in vivo following intranasal infection of mice with wild type and mutant strains of GAS that expresses the Ova antigen on their surface. Experiments will determine impact of infection on the antigen specific T cell response in NALT and other lymphoid organs.

描述（由申请人提供）：仅在美国，每年有500万人被诊断患有链球菌性咽炎，每年有1200-1500人死于A组链球菌（GAS）感染的并发症。 这个预测的总体目标是了解多达三分之一的儿童积极治疗咽炎继续摆脱链球菌的细胞和免疫学原因，以及为什么更多的儿童未能产生可测量的免疫反应。我们的研究将利用一个成熟的鼻内感染小鼠模型来模拟人扁桃体的感染。初步结果表明，GAS对BalBc鼻道下淋巴组织（NALT）具有有效的向性。转基因、初始卵清蛋白特异性CD 4 T细胞的连续转移将用于研究在用在其表面上表达Ova抗原的野生型和突变型GAS菌株鼻内感染小鼠后体内基于T细胞的免疫应答。实验将确定感染对NALT和其他淋巴器官中抗原特异性T细胞应答的影响。