Transdifferentiation Potential of Bone Marrow Stem Cells
骨髓干细胞的转分化潜力
基本信息
- 批准号:6879603
- 负责人:
- 金额:$ 31.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-04-01 至 2007-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): Recent studies demonstrated that stem cells in a number of organs/tissues have the potential to differentiate into tissues other than those specified in their origins. The most striking evidence for this "transdifferentiation" has been between bone marrow and muscular, hepatic, and CNS systems. Since all of the transplantation studies have been conducted using populations of cells, it is possible that the apparent bipotentiality of the stem cells is from engraftment by two separate populations of monopotential stem cells. Our proposal seeks definitive information on the tissue/organ engrafting capabilities of the hematopoietic and stromal stem cells by using single stem cell transplantation. We will use transgenic CD45.2 C57BL/6 (B6) mice that ubiquitously express enhanced green fluorescent protein (EGFP) as the source for donor stem cells and congenic CD45.1 B6 mice as recipients. The contribution of bone marrow EGFP-labeled donor cells to various organs/tissues of the recipient mice will be assessed using laser scanning confocal microscopy (LSCM) and conventional epifluorescence microscopy in combination with differential interference contrast (DIC) microscopy. Two Specific Aims are proposed: (1) To define the potentials of individual hematopoietic stem cells by single stem cell transplantation to lethally irradiated recipient mice. (2) To define the potentials of stromal stem cells. We have developed a highly efficient technique to create mice with clonal hematopoietic engraftment by combining FACS cell sorting and short-term suspension culture of bone marrow cells. Collaboration with the laboratory of Dr. Christopher J. Drake of Department of Cell Biology and Anatomy, who has considerable expertise in the use of LSCM and DIC and epifluorescence microscopy, has already resulted in exciting preliminary information about the potentiality of a single EGFP-labeled stem cell.
描述(由申请人提供):最近的研究表明,许多器官/组织中的干细胞具有分化成其来源指定组织以外的组织的潜力。这种“转分化”最显著的证据出现在骨髓和肌肉、肝脏和中枢神经系统之间。由于所有的移植研究都是使用细胞群进行的,因此干细胞的明显双电位可能是由两个单独的单电位干细胞群植入的。我们的建议是通过使用单个干细胞移植寻求关于造血干细胞和基质干细胞的组织/器官移植能力的明确信息。我们将使用普遍表达增强绿色荧光蛋白(EGFP)的转基因CD45.2 C57BL/6 (B6)小鼠作为供体干细胞的来源,并使用同源CD45.1 B6小鼠作为受体。骨髓egfp标记的供体细胞对受体小鼠各种器官/组织的贡献将通过激光扫描共聚焦显微镜(LSCM)和传统的荧光显微镜结合差干涉对比显微镜(DIC)来评估。提出了两个具体目标:(1)通过单个干细胞移植到致死性辐照受体小鼠来确定个体造血干细胞的潜力。(2)明确基质干细胞的潜能。我们开发了一种将FACS细胞分选和骨髓细胞短期悬浮培养相结合的高效克隆造血移植小鼠技术。与细胞生物学和解剖学系Christopher J. Drake博士的实验室合作,他在LSCM、DIC和荧光显微镜的使用方面具有相当的专业知识,已经产生了关于单个egfp标记干细胞潜力的令人兴奋的初步信息。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Makio Ogawa其他文献
Makio Ogawa的其他文献
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{{ truncateString('Makio Ogawa', 18)}}的其他基金
Tissue Reconstituting Potential of Human Stem Cells
人类干细胞的组织重建潜力
- 批准号:
7046860 - 财政年份:2005
- 资助金额:
$ 31.5万 - 项目类别:
Tissue Reconstituting Potential of Human Stem Cells
人类干细胞的组织重建潜力
- 批准号:
6924833 - 财政年份:2005
- 资助金额:
$ 31.5万 - 项目类别:
Tissue Reconstituting Potential of Human Stem Cells
人类干细胞的组织重建潜力
- 批准号:
7391576 - 财政年份:2005
- 资助金额:
$ 31.5万 - 项目类别:
Tissue Reconstituting Potential of Human Stem Cells
人类干细胞的组织重建潜力
- 批准号:
7216721 - 财政年份:2005
- 资助金额:
$ 31.5万 - 项目类别:
Transdifferentiation Potential of Bone Marrow Stem Cells
骨髓干细胞的转分化潜力
- 批准号:
6611834 - 财政年份:2003
- 资助金额:
$ 31.5万 - 项目类别:
Transdifferentiation Potential of Bone Marrow Stem Cells
骨髓干细胞的转分化潜力
- 批准号:
7030918 - 财政年份:2003
- 资助金额:
$ 31.5万 - 项目类别:
Transdifferentiation Potential of Bone Marrow Stem Cells
骨髓干细胞的转分化潜力
- 批准号:
6717692 - 财政年份:2003
- 资助金额:
$ 31.5万 - 项目类别:
PHYSIOLOGICAL ROLE OF ETS GENES IN HEMATOPOIESIS
ETS 基因在造血中的生理作用
- 批准号:
6478158 - 财政年份:2001
- 资助金额:
$ 31.5万 - 项目类别:
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