Selective Breeding for Ethanol Sensitivity

乙醇敏感性的选择性育种

• 批准号: 6966939
• 负责人: RICHARD A DEITRICH
• 金额: $ 22.9万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-18 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6966939
• 关键词: Selective; Breeding; Ethanol; Sensitivity

DESCRIPTION (provided by applicant): This is a supplemental application to take advantage of a highly significant Quantitative Trait Locus (QTL) on chromosome 1 for high dose ethanol sensitivity that we have discovered in the Inbred Alcohol non- Tolerant (IANT) and the inbred Alcohol Tolerant (IAT rats) in spite of the fact that these animals were selectively bred for low dose effects. This QTL is in the same general location as one for this phenotype in the inbred High Alcohol Sensitive (IHAS) and inbred Low Alcohol Sensitive (ILAS) rats, but is much more significant and has a much higher heritability. We will take advantage of this unique opportunity to add to our knowledge of ethanol sensitivity by construction of congenic strains for the QTL, narrowing the region of the QTL and comparing the results to the same region in the I HAS and I LAS rats. We are also constructing congenic strains from the I HAS and I LAS rats on chromosomes 2 and 5. The QTL on chromosome 1 in the IHAS and ILAS rats is much smaller than in the IANT and IAT rats. These studies are important since among the risk factors for alcoholism in humans is a low initial sensitivity and/or rapid development of tolerance to ethanol. We have two animal models of this behavior, the rats selectively bred for high dose sensitivity (the IHAS and ILAS) and animals bred for low dose sensitivity (the IAT and IANT)

描述(由申请人提供)：这是一个补充申请，以利用1号染色体上高剂量酒精敏感性的一个高度显著的数量性状基因座(QTL)，这是我们在近交系不耐酒精(IANT)和近交系耐酒精(IAT)大鼠中发现的，尽管这些动物是为低剂量效应而选择性繁殖的。该QTL与近交系高酒精敏感(IHAS)和近交系低酒精敏感(ILAS)大鼠的这一表型的QTL位于相同的位置，但更显著，遗传力更高。我们将利用这一独特的机会，通过构建QTL的同源菌株，缩小QTL的区域，并将结果与I HAS和I LAS大鼠的相同区域进行比较，来增加我们对酒精敏感性的了解。我们还在2号和5号染色体上构建了IHAS和ILAS大鼠的同源株系。IHAS和ILAS大鼠1号染色体上的QTL比IANT和IAT大鼠小得多。这些研究很重要，因为人类酒精中毒的危险因素之一是对酒精的低初始敏感性和/或快速发展的耐受性。我们有两种这种行为的动物模型，一种是选择性饲养的高剂量敏感性的大鼠(IHAS和ILAS)，另一种是低剂量敏感性的动物(IAT和IANT)。