Transcription Factor Function in Flower Development

转录因子在花发育中的功能

• 批准号: 6881848
• 负责人: NICOLE J. KUBAT
• 金额: $ 0.96万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6881848
• 关键词: Arabidopsis; DNA methylation; cell cell interaction; chromatin; cytogenetics; developmental genetics; gene induction /repression; genetic mapping; genetic transcription; genetically modified plants; high throughput technology; histones; molecular dynamics; plant genetics; plant growth /development; plant morphology; plant proteins; polymerase chain reaction; postdoctoral investigator; protein localization; protein structure function; site directed mutagenesis; transcription factor

DESCRIPTION (provided by applicant): While a number of transcription factors involved in floral development have been identified, the exact mechanisms by which many of these transcription factors function are unknown. The experiments in this proposal aim to elucidate the molecular mechanisms by which two flower specific transcription factors function to regulate transcription during flower development. One of these transcription factors, encoded by the APETALA3 (APS) gene is a floral organ identity gene important for both petal and stamen development, and the other, SUPERMAN (SUP), is important for boundary formation between two specific floral domains, or whorls. The objective of the proposed experiments is to elucidate how APS and SUP function at the molecular level to mediate transcriptional activation and repression of target genes, respectively. Specifically, results will indicate whether APS- and SUP-mediated transcriptional regulation involves chromatin- and/or epigenetic-level changes, as well as elucidate APS and SUP co-factors involved in the process of transcriptional regulation.

描述（由申请人提供）：虽然已经鉴定了许多参与花发育的转录因子，但是这些转录因子中的许多转录因子起作用的确切机制是未知的。本实验旨在阐明两种花特异性转录因子在花发育过程中调控转录的分子机制。这些转录因子之一，由APETALA 3（APS）基因编码，是一个花器官身份基因，对花瓣和雄蕊发育都很重要，另一个，SUPERMAN（SUPERMAN），对两个特定的花域或轮生之间的边界形成很重要。本实验的目的是阐明APS和BMPs如何在分子水平上分别介导靶基因的转录激活和抑制。具体而言，结果将表明APS和SUP介导的转录调控是否涉及染色质和/或表观遗传水平的变化，以及阐明APS和转录调控过程中涉及的辅助因子。